Drive of Clio or Thalia

Control of two CellMembra or CellRetention P-SUBs

Clotho is a dual channel Drive Unit for two independently operating; Clio diaphragm OneWay-Single-Use-Pump's like integrated with CellMembra P-SUB; or Thalia diaphragm Alternating-Single-Use-Pump's like integrated with the CellRetention P-SUB. Both for cultivating mammalian cells in a Semi-Continuous Process.


Product, model name



Dual SUP/HFF control

Drive gas pressure supply

>1.5 - <4 bar atmospheric

Drive gas vacuum supply

<0.1 bar absolute

Power supply

24 VDC, 2 amp

Hephaestus cabinet size


Weight, kilo



Clotho-2 software is loaded into the ultra compact Drive Unit maximizing the performance of Clio type of One-way-Single-Use-Pump and the Thalia Alternating-Single-Use-Pump.

Clio and Thalia SUPs is integrated with a Single-Use-Bioreactor (SUB) for high density cell cultivation operating in perfusion mode. Such as the CellMembra or CellRetention products. The P-SUBs integrates either the Clio or Thalia (O-SUP or A-SUP), the Hollow-Fiber-Filter (HFF), and the Single-Use-Sensor's (SUS) - all in one package ready to use. Clotho Drive Unit control accurately the pumped/exchanged volume and the velocity of the feedstock passing along the HFF.

Driving Clio or Thalia requires pressurized air and vacuum. The drive gas pressure and mass-flow are continuously adjusted by sets of proportional valves inside Clotho. The intelligent software with advanced PID algorithms gets information from LASER and pressure sensors.

Front panel of Clotho-2 /dual channel model in Hephaestus Stainless Steel U2 cabinet shown on top of Cilix-B2 motor driver. The middle photo show the Drive Unit including the two red LASER sensor's. Further Clotho-2 assembled with one Sarpedon reservoir and two Alagonia pumps in one compact tower. This fully independent setup ready to use right out of the box.


When Clotho is connected to gas supply from both a pressure and vacuum source with sufficient capacity, then the spec is:

  • Drive of two Clio or Thalia size M80 or M100 simultaneously
  • Drive of one Thalia-M140 with both channels combined
  • Pumped volume (Cardiac Output/CO) according to stroke length and size
  • Stroke length, %: 1 – 100
  • Clotho inform about velocity according to HFF info input, m/s: 0.1 – >10

Please check:

Supply gas is defined as: 1 - "Supply gas pressure" and; 2 - "Supply gas vacuum". Sufficient supply is when the volume (measure in "normal liter/min") of both pneumatic pressure and vacuum quality is 1-2 times the planned CO in "nl" at specified pressures. 

Suitable supply gas pump's, also in Hephaestus cabinet's are Alagonia. Check also out the Sarpedon reservoir stabilizing pressure/vacuum and reduce pulsation when several SUPs operate on the same source of supply gas.

Clotho can off course be driven by in lab installed pneumatic air and sufficient vacuum capacity.


  • Internal 5” touch sensitive TFT display or external to PADs or smartPhones via Wi-Fi for programming and info from the built-in webserver
  • USB port for USB adapter/Wi-Fi connection 
  • RJ45 for IP/TCP via LAN and ModBus / OPC-UA communication
  • 24 VDC inlet via one Buccaneer 3 pole connector
  • LASER class 2 sensor, 650 nm wavelength, <1.2 milli Watt power, IP67 - see safety notice under Download/Manuals

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