100 ml Working Volume P-SUB with A-SUE designed for the Clotho Drive Unit

World first small Perfusion-SUB 500 ml Vessel Volume (VV) with SUB dimension OD 82 x 120 mm. Combined with the Thalia-30 A-SUE for alternating exchange of the broth flow along the 0,2 µm pore size Hollow-Fiber-Filter module (HFF). Quite advanced with both pH SUS, DO SUS and even bio-mass Single-Use-Sensor all of classical signal.

From left: Illustration show relevant fluid schematics with Magnetic-Bottom-Drive P-SUB, controlled diaphragm A-SUE and traditional HFF. Complete setup for CellRetention-500 in Heating-Support-Foot (HSF-E). Thalia driven by Clotho Drive Unit and air / vacuum Alagonia pump's / Sarpedon reservoir. Photo show the same setup with Clotho mounted vertically.

From left: CAD rendered illustrations show complete and ready to use CellRetention-mini P-SUB with one pitch blade impeller. 4 head plate port for; VisiWell, OneFerm, PICO biomass and level sensor. Photo of the same setup with finished products and P-SUB mounted in HSF-E. Cad rendered drawing illustrating the A-SUE mounted vertically for lowest possible in-balance.

From left: Thalia-30 mounted horizontal in LFT-30 also supporting red Laser sensor from underneath for on-line measuring the position of the diaphragm and hereby full control of both velocity and volume via Clotho Drive Unit. Close-up of the 4 SUS mounted vertically through head plate. VisiFerm is your own 120 mm RUS inserted into VisiWell (SUW).

SUB to support the high cell density cultivation:

  • Configurable design – if you have special request to impeller design and hose configuration
  • Agitation via Magnetic-Bottom-Drive (MBD) on Magnetic-Stirrer-Table (MST)
  • Electrical or liquid thermal control integrated all within the Heating-Support-Foot (HSF)

HFF – harvest continuous a cell free permeate

PerfuseCell as standard offer a dry stored 0,2µm pore size HFF. You can though choose from one of your preferred HFF suppliers. We pre-install whichever HFF you want. We can even install two HFF in parallel – each in series with a manual valve for choice of use.

Thalia-30 A-SUE = Alternating-Single-Use-Exchanger:

  • The A-SUE exchange broth from the SUB through the HFF and return to the SUB through the HFF -simultaneously the permeate pass the HFF barrier as harvest
  • Thalia-30 A-SUE is integrated with both the SUB and the HFF
  • Thalia-30 A-SUE take advantage of the re-usable Laser sensor support
  • The Laser sensor measures in real-time with high precision the position of the diaphragm for volume exchange and broth velocity calculation performed by Clotho
  • Thalia-30 A-SUE can exchange any volume within its spec (Cardiac Output) with programmable velocity from 0,1 to +10 m/sec through the HFF

SUS - Single-Use-Sensor's - pre-installed as standard:

  1. Port occupied by VisiWell for the VisiFerm 120 mm size DO sensor digital or analogue type
  2. Port occupied by FermProbe pH PG 13,5 x 120 classical analogue signal via S8 connector
  3. Port occupied by Futura-PICO capacitance bio-mass Single-Use-Sensor
  4. Port occupied by broth exchange tube and one level sensor + ground with 2 mm banana plugs

P-SUB – perfusion controlled by the Clotho Drive Unit:

  • CellRetention-mini™ P-SUB work excellent with most Process-Control-Systems (PCS)
  • CellRetention-mini broth exchange is performed by Thalia Alternating-SUE
  • Drive Unit is designed to handle one or two Thalia-30 Single-Use-Exchanger’s and ensure efficient and reproducible operation
  • Clotho easy programmable broth/retentate velocity and volume through HFF module for harvest and for removal of membrane deposits
  • Connection to compact pressurized air and vacuum unit's like the Alagonia and Sarpedon - if you don't have in lab pressurized air and vacuum

World smallest Perfusion-SUB able to be operated from any PCS.

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