400 - 2.000 ml Working Volume P-SUB with A-SUE to be driven by Clotho Drive Unit
PerfuseCell offer all pre-assembled 3.200 ml Vessel Volume CellRetention-3200. SUB size OD 137 x 270 mm combined with the Thalia-100 A-SUE and Hollow-Fiber-Filter and SUS – ready to use.
From left: Illustration show fluid diagram with Magnetic-Bottom-Drive SUB, controlled diaphragm A-SUE and passive HFF. The customizable SUB suction tube connects via a hose to the HFF top and opposite of HFF to the A-SUE sanitary coupling. The A-SUE is mounted on a foot, which contain the red Laser sensor for real-time measuring the position of the diaphragm and hereby full control of velocity and selected exchange volume.
From left: Pre-assembled A-SUE with sanitary coupling and bottom of HFF visible. The pre-assembled A-SUE/HFF is mounted on the LFT-100 foot which also house the red Laser sensor for real-time measuring the position of the diaphragm. 3 – Thalia-100 A-SUE next to the 3,2 liter SUB with visible impeller and Magnetic-Bottom-Drive Aero motor for agitation.
P-SUB - for cultivation (the SUB is sourced from sister company CerCell):
- 100% configurable design for the 3,2 liter Vessel Volume (VV) - Working Volume (WV) as low as 400 ml
- Agitation from bottom by Magnetic-Bottom-Drive (MBD) driven by the Magnetic-Stirrer-Table (MST-U)
P-SUB - Thermal Control
- Electrical blanket heating - wrap the orange silicone blanket around the SUB - (dim 75 x 350 mm) - traditional
- Electrical jacket heating - Heating-Support-Foot integrated with the Magnetic-Stirrer-Table (MST) - the HSF adds important stability and ease in use - best choice
- Water Re-Usable-Jacket (RUJ) for heating/cooling even combined with the Magnetic-Bottom-Drive (MST)
HFF – harvest continuous a cell free permeate
PerfuseCell as standard offer a dry stored 0,2 µm pore size HFF. You can though choose from one of your preferred HFF suppliers. We pre-install whichever HFF you want. We can even install two HFF in parallel – each in series with a manual valve for choice of use.
Thalia-100 A-SUE = Alternating-Single-Use-Exchanger:
- The diaphragm Alternating-Single-Use-Exchanger (A-SUE) exchange back and forth broth from the SUB through the HFF
- The A-SUE fits onto the re-usable Laser-Foot-Thalia
- The high precision Laser sensor measures in real-time and accurate the position of the diaphragm so broth velocity and exchanged volume calculation can be performed
- Thalia-100 can exchange any volume within its spec programmable from 0,1 to +10 m/sec velocity through the HFF
- Thalia exchange equally well gas or liquids or any mix
SUS - Single-Use-Sensor's to choose from:
- CellRetention™ features pre-installed 225 mm long SUS for DO, pH, level/ground as standard
- Also available with Futura PICO bio-mass SUS mounted horizontal at the bottom of the 3,2 liter SUB
- SUS combines with most PCS cabling and the FUTURA PICO bio-mass sensor amplifier
- Completely eliminate the need for components sterilized in conventional autoclave
From left: Relevant presentation, Cronus tower (shown Clotho-dual Drive Unit and Sarpedon) setup for drive of the A-SUE (except the PCS for control of the SUB cultivation process). Details showing the red Laser sensor in the Laser-Foot, the P-SUB mounted on MST-C driven by a Kollmorgen servo motor. On top of the A-SUE visible the 14 straw HFF with sanitary coupling.
A-SUE – Thalia operated by the Clotho Drive Unit:
- CellRetention™ P-SUB works with any PCS
- Drive Unit Clotho designed to control the Thalia Single-Use-Exchanger and ensure efficient and reproducible operation
- From Clotho programmable broth/retentate velocity and volume through HFF module for harvest and for removal of potential membrane deposits
- Connection to pressurized air and vacuum within your lab - or from unit's like Alagonia and Sarpedon - if you don't have access to pressurized air and vacuum
- Super compact Clotho available in either single or dual channel models