Clio pump features
Clio is a real pump for uni-direction fluid pump and part of CellMembra perfusion concept. Build for super controlled and selectable operation by the advanced Clotho Drive Unit. Clotho measures accurately in real-time the free-flowing diaphragm position. Allowing Clotho programming the desired diaphragm position and function. The result is an Air-Operated-Diaphragm-positive-Displacement (AODPD) One-way-Single-Use-Pump (O-SUP) with un-parallel performance and capabilities.
Clio technical / functionality:
- The diaphragm Single-Use-Pump (O-SUP) integrates two passive one-way valves guiding the direction of broth flow. The valve body receives broth from the SUB and deliver feedstock to the Hollow-Fiber-Filter (HFF) module inlet after which the concentrated broth passes axially the HFF cartridge and exit as retentate to be returned to the SUB.
- Clio O-SUP integrates with both the SUB and HFF hereby operating in a loop.
- Clio feature the re-usable (red) Laser sensor reading through the transparent Clio dome wall.
- The high precision Laser sensor measures the position of the diaphragm, so broth velocity and volume calculation can be performed and presented on the Clotho display in real-time.
- Clio both pump and measures any volume ranging within its spec and from 0 to +10 m/s velocity through the Cross-Flow-Filter module.
User features:
- Clio handle fluids = liquids and / or gases or any mix of liquid/gas
- Clio is a true metering feed pump = Positive Displacement (PD) pump = accurate mass-flow measuring at any time
- Clio O-SUP do NOT need any mass-flow sensor measuring the mass-flow
- Clio easily sucks from low arranged containers
- Clio is a low shear force design which do not damage mammalian cells
- Clio pump do NOT need calibration like peristaltic pumps
- Clio pump do NOT loose nano size silicone particles to the fluid like peristaltic pumps
Clio general specifications:
|
Product, variant names |
Clio-30 |
Clio-100 |
Clio-300 |
Clio-500 |
|
Layout, number of diaphragms |
single |
single |
single |
single |
|
Diaphragm diameter, mm |
62 |
80 |
100 |
125 |
|
Diaphragm stroke, mm |
25 |
39 |
46 |
54 |
|
Stroke Volume, SV, mL/S, 100% stroke |
34 |
100 |
500 |
500 |
|
Cardiac Output, CO range, mL/min (recommended) |
0-500 |
0-1.000 |
0-2.000 |
0-3.000 |
|
Beats-per-Minute, BpM, max |
0-22 |
0-20 |
0-15 |
0-13 |
|
Typical hose connection, ID/OD mm |
4/8 |
8/12 |
12/18 |
12/18 |
|
SUP cell weight, grams |
100 |
200 |
300 |
400 |
The basics in operation is copied from the human heart functionality:
- Cardiac Output (CO) = total pumped volume, SUP capacity, mL/min (CO = BpM x SV)
- Beats-per-Minute (BpM) = ranging 0 - 30 BpM, SUP diameter/stroke volume depending
- Stroke Volume (SV) = programmable from 10 - 100% of SUP specification
Physical features
- No expensive housing materials for traditional steam cleaning requirements
- No mechanical rotating parts such as electrical motor, coupling, rollers or gearing
- No moving ball valves, but simple cross-slit valves
- All single-use plastic parts supplied irradiated – contamination not possible
- All pumps offer infinite turn-down, turn-up scale better than 1:10.000