World only mini Perfusion-SUB integrating both HFF, A-SUE and SUS

Mini P-SUBs for cell retention in perfusion cultivation setup in a fully single-use setup. The Pulsating-Tangential-Flow (PTF) which integrate a Single-Use-Bioreactor (SUB) and the Alternating-Single-Use-Exchanger (A-SUE) and Single-Use-Sensor's (SUS) and the Hollow-Fiber-Filter (HFF) opens up for new opportunities.

General features of CellRetention-500 P-SUB:

• No space consuming servo motor attached to the head plate - take advantage of Magnetic-Bottom-Drive (MBD)
• Designed with 4 x head plate ports and 6 ports for In&Out with small diameter hoses in weldable length 
• Exchanged broth volume fully programmable and obtained velocity all accurately measured – no guessing
• Spectrum HFF with pore 0,2 µm, 14 x 1,0 mm PES straw/lumen, 88 cm2 area, Luer-Lok side ports, (D02-P20U-10-N)
• The complete and pre-assembled P-SUB unit packed in dual film bags and precision irradiated – forget the autoclave
• SUB dimension is OD 82 x 120 mm for Working Volume (WV) ranging from 100 ml up to 250 ml
• Thermal control either electrical or water – Heating-Support-Foot available for 48 VDC, for 110 VAC, for 230 VAC - or for water

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CellRetention-500 complete

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CellRetention-500 in HSF and Clotho

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CellRetention-500 SUS details

Right photo show with VisiFerm installed the position of the VisiWell (VisiFerm not included). Center photo P-SUB mounted in Heating-Support-Foot (HSF-E-230). The HSF helps stabilize the un-balanced weight distribution and improve temperature accuracy. Red Laser sensor mounted in a Re-Usable-Bracket above the dome of the Thalia-30 A-SUE. Clotho controller require only the cable to the Laser sensor and the blue pneumatic hose to drive the A-SUE. Right photo show vertical arranged Thalia directly coupled to the HFF for minimum dead volume.

Further P-SUB size 500 features:

• Comes with Magnetic-Bottom-Drive (MBD) for up to 250 RPM on Magnetic-Stirrer-Table (MST)
• Pitch blade impeller - you an choose blade design, number blades, angle, diameter, etc
• 2 x small diameter weldable hoses length 600 mm
• 1 x small short hose with 5 cm2 sterile filter for aeration via a 20 µm micro porous sparger body
• 1 x small short hose with swabable valve for bottom sampling
• 1 x exhaust hose with 16 cm2 sterile filter 
• Need for liquid thermal control = Heating-Support-Foot (HSF-W)
• Need for electrical thermal control = Heating-Support-Foot (HSF-E-voltage)

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SUS in CellRetention-500 closeup

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Thalia-30 sanitary small HFF

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Thalia-30 HFF on top

DO, pH, PICO, level SUS mounted in the 4 port head plate. If needed stand alone Thalia A-SUE fixed into the black LTF-30 which also support the red Laser sensor.

The 4 available head plate ports are used for:

1. One port occupied by OneFerm or FermProbe pH Single-Use-Sensor (SUS) S8 classical signal
2. One port occupied by VisiWell Single-Use-Well (SUW) designed for VisiFerm (RUS) 120 mm DO sensor
3. One port occupied by PICO bio-mass Single-Use-Sensor vertical mounted
4. One port occupied by SuctionTunk with siphon tube and selectable ml level sensor (100, 150, 200, 250 ml) for PCS connection