100-250 ml Working Volume P-SUB designed for the Clotho drive unit
Smallest perfusion SUB we currently offer is 500 ml VV with vessel dimension OD 82 x 120 mm. Combined with the early Clio-30 O-SUP for one-way flow path of the broth, and 0.2 µm pore Hollow-Fiber-Filter (HFF) module. Quite advanced with both pH and DO and bio-mass Single-Use-Sensor's of classical signals.
CellMembra fluid layout
Cronus-Tower + CellMembra 500 ml
4 x PG 135 ports on CellMembra 500
From left illustrate fluid schematics with Magnetic-Bottom-Drive P-SUB, controlled diaphragm SUP and HFF. Centre photo complete setup for CellMembra-mini in Heating-Support-Foot (HSF) driven by Clotho-1 controller and air / vacuum Alagonia pumps / Sarpedon reservoir. Right details to SUS.
CAD rendered illustrations show complete and ready to use CellMembra-mini 500 ml P-SUB with one pitch blade impeller. 4 port for VisiWell, OneFerm, PICO biomass and Suction Trunk connecting the Clio-30 diaphragm SUP.
From left show 500 ml P-SUB mounted with pre-installed OneFerm pH and VisiFerm inserted into the VisiWell. Centre photo show two HSF and the two P-SUBs one 500 ml and one 300 ml illustrate limited size difference. Right photo show the red Laser sensor for on-line measuring the position of the diaphragm and hereby full control of both velocity and volume via Clotho Drive Unit.
SUB for the cultivation:
- Configurable design – if you have special request to impeller design and hose configuration
- Agitation via Magnetic-Bottom-Drive (MBD) mounted on Magnetic-Stirrer-Table (MST)
- Electrical heating or water jacket thermal control integrated within the Heating-Support-Foot (HSF)
HFF – harvest continuously a cell free permeate. Choose from one of your preferred HFF suppliers, such as:
- HFF from www.repligen.com
- Cytiva
- HFF from www.parker.com/dh-bioprocessing
We pre-install whichever HFF you want.
Clio-CM80 O-SUP = One-way-Single-Use-Pump:
- The diaphragm O-SUP integrate two passive one-way valves guiding the direction of broth flow. The O-SUP valve body receive broth from the SUB, the O-SUP force the feedstock through the HFF, after which the retentate is returned to the SUB and permeate pass the HFF membrane as harvest
- Clio CM80 O-SUP is integrated with both the SUB and HFF
- Clio CM80 take advantage of the re-usable LASER sensor supported
- The LASER sensor measures in real-time and with high precision the position of the diaphragm so broth velocity and volume calculation can be performed by Clotho
- Programmable Clio CM80 can pump any Cardiac Volume - see spec - and from 0 to +10 m/sec velocity through the HFF module
SUS - Single-Use-Sensor's - pre-installed as standard:
- Port occupied by VisiWell for the VisiFerm 120 mm size DO sensor digital or analogue signal
- Port occupied by FermProbe pH PG 13.5 x 120 classical analogue signal via S8 connector
- Port occupied by ABER-PICO capacitance bio-mass Single-Use-Sensor (limited availability)
- Port occupied by the Suction Trunk (broth out & retentate return to Clio) including level sensor for dual 2 mm banana plugs
SUB – perfusion controlled by the Clotho Drive Unit:
- CellMembra™ Perfusion-SUB work excellent with most Process-Control-Systems (PCS)
- CellMembra broth circulation is performed by Clio O-SUP
- O-SUP Drive Unit, named Clotho, is designed to handle one or two Clio-M80 Single-Use-Pump's and ensure efficient and reproducible operation
- From Clotho programmable feedstock velocity and volume through HFF module for harvest and for removal of membrane deposits is easy to setup
- Connection to compact pressurized air and vacuum unit's like the Alagonia and Sarpedon - if you don't have in lab pressurized air and vacuum
World smallest perfusion SUB able to be operated by most PCS.