250 ml mini P-SUB with SUP, HFF on Modular PCS
Perfusion-SUBs for ATF operation method offers:
- Fouling free HFF (Hollow-Fibre-Filter) perfusion process
- Innovative LASER guided Single-Use-Pump (SUP) diaphragm pump
- Outstanding Clotho software - stand-alone or OPC-UA connected
- 80 – 150 ml Working Volume with 250 ml SUBs
- Pre-assembled assembly with SUP, deep tube and HFF as needed
Clotho drive unit integrates pressures, vacuum, volume sensor's eliminating the need for external sensor's for TMP, pressure drop, flow measurements.
Clotho process software controls accurately the stroke volume, the number of strokes, the allowed pressure drop along the HFF for fully automatic harvest and fully selectable fluid velocity for the sequential HFF cleaning eliminating HFF blockage.
Left photo show two 250 ml SUBs connected to short and long HFF and the LASER guided Thalia SUP next to the Clotho drive unit. The right photo shows one 250 ml SUB connected via a hose to HFF on top of Thalia with the red LASER sensor under the SUP. Note the is NO flow restricting Luer-Loc in the broth path for lowest possible shear stress.
General features of CellRetention combined with the 250 ml SUB for Modular operation
- Combining the 250 ml batch SUB with HFF and Thalia for trouble free operation
- Thalia™ LASER guided Single-Use-Pump TM80/30 controlled independent by outstanding Clotho™ software
- Alternating broth volume and velocity fully programmable, accurately measured and fully repeatable – no process guessing
- Direct-Fit HFF with 0.2 µm pore and length from 200 to 600 mm eliminating shear stress caused by typical Luer-Loc™ fittings
- The complete and pre-assembled P-SUB packed in dual film bags and precision irradiated – forget the autoclave