100 - 250 ml ATF Perfusion-SUB

Mini size CellRetention keep HFF free from fouling


World only P-SUB for ATF method offer:

  • HFF (Hollow-Fibre-Filter) fouling free process with innovative diaphragm pump
  • 100 – 250 ml Working Volume fully pre-assembled P-SUB
  • Alternating of broth by Thalia M80 diaphragm Alternating-Single-Use-Pump (A-SUP)
  • Outstanding Clotho software - stand-alone or OPC-UA connected

Industry leading architecture P-SUB pre-installed with:

  • Direct-Fit HFF (Hollow-Fibre-Filter) eliminating the shear stress created by the tiny Luer-Loc
  • Ground-breaking LASER controlled diaphragm Single-Use-Pump (SUP)
  • Single-Use-Sensors (SUS) for DO, pH, temp, level

Right photo show with VisiFerm installed the position of the VisiWell (VisiFerm not included). Center photo P-SUB mounted in Heating-Support-Foot. The HSF helps stabilize the un-balanced weight distribution and improve temperature accuracy. Red LASER sensor mounted in a Re-Usable-Bracket above the dome of the Thalia M30 A-SUP. Clotho controllers require only the cable to the Laser sensor and the blue pneumatic hose to drive the Thalia A-SUP. Right photo show vertical arranged Thalia directly coupled to the HFF for minimum dead volume.

General features of CellRetention-500 P-SUB:

  • No space consuming servo motor attached to the head plate - take advantage of Magnetic-Bottom-Drive (MBD)
  • Designed with 4 x head plate ports and 6 ports for In&Out with small diameter hoses in weldable length 
  • Exchanged broth volume fully programmable and obtained velocity all accurately measured – no guessing
  • HFF with pore 0.2 µm, 14 x 1.0 mm PES straw/lumen, ?? cm2 area, Luer-Lok ports, (D0?-P20U-10-N)
  • The complete and pre-assembled P-SUB unit packed in dual film bags and precision irradiated – forget the autoclave
  • SUB dimension is OD82 x 120 mm for Working Volume (WV) ranging from 100 ml up to 250 ml
  • Thermal control either electrical or water – Heating-Support-Foot available for 110 VAC and for 230 VAC

Further P-SUB size 500 features:

  • Comes with Magnetic-Bottom-Drive (MBD) for up to 250 RPM on Magnetic-Stirrer-Table (MST)
  • Pitch blade impeller - you an choose blade design, number blades, angle, diameter, etc
  • 2 x small diameter weldable hoses length 600 mm
  • 1 x small short hose with 5 cm2 sterile filter for aeration via a 20 µm micro porous sparger body
  • 1 x small short hose with swabable valve for bottom sampling
  • 1 x exhaust hose with 16 cm2 sterile filter 
  • Need for liquid thermal control = Heating-Support-Foot (HSF-W)
  • Need for electrical thermal control = Heating-Support-Foot (HSF-E-voltage)

DO, pH, PICO, level SUS mounted in the 4 port head plate. If needed the otherwise standalone Thalia A-SUP can fixed to the P-SUB headplate. Center photo – Direct-Fit HFF to scalable Thalia for better flow performance and lower shear stress. The small size M80 with Luer-Lock outlet on the right side.

The 4 available head plate ports are used for:

  1. One port occupied by OneFerm or FermProbe pH Single-Use-Sensor (SUS) S8 classical signal
  2. One port occupied by VisiWell Single-Use-Well (SUW) designed for VisiFerm (RUS) 120 mm DO sensor
  3. One port occupied by PICO bio-mass Single-Use-Sensor vertical mounted - according to availability from ABER. (not available for the time being).
  4. One port occupied by SuctionTunk with siphon tube and selectable ml level sensor (100, 150, 200, 250 ml) for PCS connection