0.5 – 1.5 liter Working Volume ATF-SUBmerged Perfusion-SUB

SUBmerged™ ATF P-SUB size 2 liter VV keep HFF free from fouling

Benefits of this particular P-SUBmerged are:

avoid HFF fouling – freedom to select broth velocities for gentle harvest and sequential pulses flushing out debris from inside the HFF
eliminate dead volume – skip all the hoses, traditional plastic connectors and SUBmerge the HFF
avoid cell death - inverted Thalia pump arranged on top of the HFF = hereby the diaphragm pump cannot accumulate cells
reduce shear force – protect cells by eliminating hoses, fittings - SUBmerge the HFF inside the P-SUB and the inverted Thalia on top of HFF
increased Kla values - aeration via membrane for gas diffusion - 30% better via large surface area tubular pico-sparger

Industry leading architecture P-SUB pre-installed ready for use:

500 – 1.5 liter Working Volume (WV) fully pre-assembled P-SUB (2 liter Vessel Volume OD 110 mm)
Alternating broth flow by Thalia diaphragm Alternating-Single-Use-Pump (TM60 or TM80 A-SUP)
SepraPor^® HFF OD 12 mm (Hollow-Fibre-Filter) size 110, 170, 340 cm2 surface SUBmerged with direct Thalia connection (no Luer-Lok or TRI-clamps) for reduced shear stress
Inverted ground-breaking LASER guided Thalia TM60 (TM80 for 340 cm2) for diaphragm Single-Use-Pump (A-SUP)
Single-Use-Sensors (SUS) for DO, pH, (optional InCyte bio-mass on SUB wall), temp, level as needed

CellRetention-2 ATF extended KLa

CellRetention-2 ATF tight headplate

CellRetention-2 ATF on HSF in MST

All three photos show the 2 liter P-SUB with OD 4 mm thin wall silicone hose Membrane-Diffusion-Cage for 30 % better KLa values compared to micro sparger. Right photo seen mounted in the Heating-Support-Foot (HSF) bolted on the Magnetic-Stirrer-Table (MST) separating the black servo motor from the P-SUB. The HSF is a perfect thermal control with sufficient contact to the SUB surface for accurate temperature regulation. Red LASER sensor mounts in a Re-Usable-Bracket above the dome of the inverted Thalia TM60 A-SUP.

General features of CellRetention™ 2 liter P-SUB designed for Head-Plate-Drive (HPD):

Head plate with 4 x PG 13.5 circumference ports and 6 ports for In&Out with small diameter hoses, exhaust port, temp well port
Thalia SUP exchanged broth volume fully programmable from Clotho Drive Unit mastering accurately velocity, volume, number of variable strokes – no guessing
SepraPor HFF manufactured by Meissner Corp, Direct-Fit coupled for lowest possible shear stress
Thermal control with Eppendorf SciVario ThermoWell

CellRetention-2 ATF HPD PG13 kit

Thalia TM60A SUP unified with the C012 SepraPor HFF and mounted in PG13 headplate port. P-SUB inserted in ID110 mm ThermoWell for thermal control with Maxon RE30 for Head-Plate-Drive all controlled by SciVario. The Hamilton DO, pH SUS pre-installed in the head plate. Direct-Fit connected HFF to Thalia for better flow performance and lower shear stress. See this link - stress free connections

Further 2 liter P-SUB features:

One pitch blade impeller
Membrane-Diffusion-Cage comprises 3 meter pico porous silicone for efficient aeration
3 small diameter weldable hoses length 900 mm
1 gas inlet small short hose with sterile filter for aeration
permeate 900 mm weldable hose - for rinsing and permeate
1 small short hose with swabable valve for bottom sampling
Optional exhaust gas cooler
Single-Use-Sensors (SUS) for DO, pH, temp, level - optional biomass
1 exhaust hose with 26 cm²sterile filter - select the Sterile-Filter-Heater

The head plate PG 13.5 ports designed for:

One port pre-installed with the OneFerm pH Single-Use-Sensor (SUS) K8 or VP
One port pre-installed with the VisiWell Single-Use-Well (SUW) designed for VisiFerm (RUS) DO sensor
extra PG 13.5 ports
CellRetention PG13 kit
Center port options; with MBD free for a SUS, with HPD occupied by bearing assembly

Further customization – contact us

All products are patented by www.stobbe.group