Medium size CellRetention™ keep HFF free from fouling

World only P-SUB performing ATF method combines:

• Alternating broth flow by Thalia diaphragm Alternating-Single-Use-Pump (TM80 A-SUP)
• Ultra-low dead-volume between HFF and SUB bottom outlet
• Ultra-low dead-volume between HFF and Thalia SUP
• HFF (Hollow-Fibre-Filter) fouling free process with innovative diaphragm pump
• 500 – 1,500 ml Working Volume fully pre-assembled P-SUB (2,000 ml Vessel Volume)

Industry leading architecture P-SUB pre-installed with:

• HFF (Hollow-Fibre-Filter) with direct connection (no Luer-Lok or TRI-clamps) for reduced shear stress
• Ground-breaking LASER guided diaphragm Single-Use-Pump (SUP)
• Single-Use-Sensors (SUS) for DO, pH, bio-mass, temp, level

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P-SUB MST SUS Thalia HFF

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1.5 liter P-SUB Mounted in HSF

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1.5 liter P-SUB+SUS+Thalia+HFF

Right photo show with VisiWell, OneFerm, InCyte patch pre-installed (VisiFerm and InCyte RUS not included). All three photos show the 1500 P-SUB mounted in the Heating-Support-Foot bolted on the MST separating the black heavy servo motor from the SUB. The HSF is a perfect thermal control with sufficient contact to the SUB surface for temperature accuracy. Red LASER sensor mounted in a Re-Usable-Bracket above the dome of the Thalia TM80 A-SUE. Clotho controller require the cable to the LASER sensor and the blue pneumatic hose for drive gas to Thalia. Right photo show vertical arranged SKYsupport bolted onto the HFF/MST for easy support of gas cooler. This unique arrangement combining the SUG, SUP and HFF offer minimum dead volume.

General features of CellRetention™ medium size P-SUB:

• No space consuming heavy servo motor attached to the bioreactor head plate - take advantage of Magnetic-Bottom-Drive (MBD) of the P-SUB
• Designed with 5 x PG 13.5 head plate ports and 6 ports for In&Out with small diameter hoses
• Exchanged broth volume fully programmable at Clotho and obtained velocity accurately measured – no guessing
• HFF could be Meissner SepraPor XFCM20, 13 x 1.0 mm straw/lumen, 85 cm2 area, direct coupled, lower shear stress, barb side ports
• HFF could be Spectrum (D02-P20U-10-N) with pore 0.2 µm, 14 x 1.0 mm straw/lumen, 75 cm² area, direct coupled, Luer-Lok side ports
  
• The complete and pre-assembled P-SUB unit packed in dual film bags and precision irradiated – forget the autoclave
• SUB dim is OD 110 x 230 mm for 2.1 liter Vessel Volume (VV) ranging from 500 ml up to 1,500 ml Working Volume (WV)
• Thermal control with the Heating-Support-Foot (HSF) available for either 110 VAC or 230 VAC

Further P-SUB medium features:

• Comes with Magnetic-Bottom-Drive (MBD) for up to 250 RPM for use with MST
• One pitch blade impeller
• 3 small diameter weldable hoses length 600 to 900 mm 
• 1 small short hose with 5 cm² sterile filter for aeration
• 1 small short hose with swabable valve for bottom sampling
• Optional exhaust gas cooler
• 1 exhaust hose with 26 cm² sterile filter - select the Sterile-Filter-Heater
  
• Need for electrical temperature control = Heating-Support-Foot (HSF) 

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P-SUB Driven by Clotho+Harmonia

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Scalable Cellretention P-SUBs

The Hamilton DO, pH SUS mounted in the head plate and InCyte patch on the side wall. The Thalia A-SUP are fixed to the P-SUB head plate and as such a very compact solution. Direct connected HFF to Thalia for better flow performance and lower shear stress. See this link - stress free connections

The PG 13.5 ports are used for:

1. One port pre-installed with the OneFerm pH Single-Use-Sensor (SUS) K( or VP
2. One port pre-installed with the VisiWell Single-Use-Well (SUW) designed for VisiFerm (RUS) DO sensor
3. Side port pre-installed with the InCyte patch designed for the Hamilton InCyte bio-mass (RUS)
4. extra PG 13.5 ports
5. Bottom side port connection to the HFF and the diaphragm SUP

Further customization – contact us

The Thalia SUP and the CellRetention P-SUB are patented by www.stobbe.group