250 ml mini P-SUB with SUP, HFF on Modular PCS
Perfusion-SUBs for ATF operation method offers:
- Fouling free HFF (Hollow-Fibre-Filter) perfusion process
- Innovative LASER guided Thalia diaphragm Single-Use-Pump (SUP)
- Outstanding Clotho software – from stand-alone or OPC-UA connected SUP driver
- 80 – 150 ml Working Volume with 250 ml Vessel Volume SUBs
Clotho drive unit integrates pressures, vacuum, volume sensing eliminating the need for external sensor's for TMP, pressure drop, flow measurements. Clotho process software controls accurately the stroke volume, the number of strokes, the allowed pressure drop along the HFF for fully automatic harvest and fully selectable fluid velocity for the sequential HFF cleaning eliminating HFF blockage.
Left photo show two 250 ml SUBs connected to short and long HFF and the LASER guided Thalia SUP next to the Clotho drive unit. The right photo shows one 250 ml SUB connected via a hose to HFF on top of Thalia with the red LASER sensor under the SUP. Note there is NO flow restricting Luer-Loc in the broth path for lowest possible shear stress.
- Combine the 250 ml batch SUB with HFF and Thalia for trouble free operation
- Thalia™ LASER guided Single-Use-Pump TM80/30 controlled independent by outstanding Clotho™ software
- Alternating broth volume and velocity fully programmable, accurately measured and fully repeatable – no process guessing
- Direct-Fit HFF with length from 200 to 600 mm eliminating shear stress caused by Luer-Loc™ fittings - https://perfusecell.com/bioreactor-products/diaphragm-pump-series-32/hff-stress-free-connection
- The complete and pre-assembled P-SUB packed in dual film bags and precision irradiated – forget the autoclave
- Granted patents
Upside-Down position reduces dead volume significant. No cells accumulated in Thalia SUP which empty itself constantly. Minimum dead volume in the fluid path.
This unique arrangement combines the SUP with the HFF via ultra short hose for minimum dead volume. Here shown with one Repligen HFF and one Meissner HFF integrated directly with the Thalia SUP eliminating connection shear force. Dynamic Support-Grip adjustable in all 3 directions secured on the base plate all made from Stainless Steel. Find the Support-Grip part here.