0.4 - 2.5 liter ATF-SUBmerged perfusion kit

ATF-SUBmerged™ kit for 430 cm² HFF to fit the 3.2 liter VV B-SUB

The 3.2 liter Vessel Volume (VV) B-SUB (Batch-Single-Use-Bioreactor) is equipped with a ID19 mm port closed with a plug in the head plate and thus able to operate as a Batch-SUB or Perfusion-SUB. When this unique B-SUB needs to operate as a P-SUBmerged the "CellRetention-3-kit-x" is easily inserted though the ID19 mm port under the hood by end-user. Remember to pre-wett the HFF and ready to use.

SUBmerged C112 TM80S kit

SUBmerged C112 TM80A kit

Talia Straigth and Angled demo

Two different "CellRetention-3-kit-x" delivered bagged and E-beam irradiated ready to be inserted in the 3 liter B-SUB through the ID19 mm port in the hood and thus become CellRetention P-SUBmerged. Both the Straight and the Angled Thalia is a Direct-Fit with the SepraPor C112-xx1x - 0.2 µm pore HFF with 430 cm2 surface. Choose Straight or Angled Thalia as needed from Commerce. Third photo illustrates a demo of the Straight for MST drive and a demo of the Angled for HPD drive with a Teknic servo motor on head plate.

Inside the B-SUB:

Aeration by super-efficient 25 or 100 µm pore glass micro sparger for high cell density, deep-tubes, addition, harvest, baffles, etc – see doc at download
CellRetention-3-kit-x DYI insert - SepraPor XFCM20C112-xx1x HFF (Hollow-Fibre-Filter) with 420 cm² surface and HFF inlet SUBmerged directly into the broth
Agitation with triple Kidney blade, OD 60 mm, 45° blade angled
Single-Use-Sensors (SUS) for DO, pH, bio-mass, temp, level as needed

Outside the B-SUB:

HPD (Head-Plate-Drive) or MBD (Magnetic-Bottom-Drive)
900 mm weldable M-Flex hose connected to various in&Out
Thermal control by liquids - equipped with Single-Use-Jacket (SUJ) (InCyte not possible)
Thermal control by Electrical-Heating-Blankets (EHB)

The B-SUB kit consists of:

0.4 – 2.5 liter Working Volume (WV) from 3.2 liter Vessel Volume (VV) (dim OD137 x 245 mm) fully pre-assembled and ready to install with known impeller Power Numbers
The CellRetention-3 B-SUB is dual foil bag packed and precision E-beam irradiated
Don’t forget to pre-wet HFF as usual – info found here – Tips&Tricks

SUBmerged 3 liter ATF HFF Cronus

SUBmerged SepraPor Thalia TM80A

CellRetention-3 B-SUB HPD MST

FlowChart connects the P-SUBmerged to the more advanced Cronus-PCS running Lucullus SCADA software. Rendered CAD illustrate the 3 liter SUBmerged with OD19 mm HFF and angled Thalia TM80A. B-SUBs available with liquid or electrical thermal control. Right photo show the ID19 mm port with the plug for removal and insert of the 430 cm2 HFF.

Clotho Drive Unit is required and integrates pressures, vacuum, volume sensing eliminating the need for external sensors for HFF DeltaP / ∆P pressure drop and mass-flow measurements. Clotho process software controls accurately the programmable stroke volume, number of strokes, the allowed pressure drop along the HFF for fully automatic broth low harvest velocity and fully selectable broth high velocity for the sequential lumen cleaning eliminating HFF blockage.

Under sterile conditions, remove the plug and nut from the B-SUB port, insert the HFF incl the new nut and O-ring fully into the ID19 port, insure the threads engage, tighten the nut so the HFF hardly can rotate.

ATF kit TM80S HFF C112+spanner

ATF kit spanner on ID19 nut

CellRetention-3 equipped RE30

If your fingers are not strong enough to tighten sufficient the nut use a spanner. Right photo illustrate that even a crowded head plate can allow space for a slimline servo motor for Head-Plate-Drive (HPD) and three PG13 ports with SUS VP and ARC adapter.

All products are patented by www.stobbe.group