Which SUP / HFF volume ration insuring sufficient velocity during HFF cleaning
Which ratio between SepraPor and Thalia to select ? The entire P-SUB ATF portfolio follow this chart.
|
|
OD mm |
total |
HFF |
HFF |
HFF Hold-Up |
Thalia stroke |
Selected SUP |
effective |
|
SepraPor |
diameter |
length |
Lumen |
surface |
volume |
volume |
Thalia |
ratio |
|
part # |
mm |
mm |
pcs |
cm2 |
cm3/ml |
cm3/ml |
model |
Thalia cm3 / HFF cm3 |
|
C012-XX1X |
9.5 |
300 |
13 |
110 |
3 |
20 |
TM60 |
7 |
|
C024-XX1X |
9.5 |
600 |
13 |
220 |
7 |
20 |
TM60 |
3 |
|
C032-XX1X |
12.0 |
300 |
25 |
205 |
5 |
20 |
TM60 |
4 |
|
C044-XX1X |
12.0 |
600 |
25 |
420 |
12 |
50 |
TM80 |
4 |
|
C112-XX2X |
19.1 |
300 |
55 |
430 |
13 |
50 |
TM80 |
4 |
|
C124-XX2X |
19.1 |
600 |
55 |
932 |
26 |
200 |
TM100 |
8 |
|
C212-XX2X |
25.4 |
300 |
115 |
938 |
26 |
200 |
TM100 |
8 |
|
C224-XX2X |
25.4 |
600 |
115 |
1949 |
54 |
200 |
TM100 |
4 |
|
C312-XX3X |
31.8 |
300 |
150 |
1224 |
35 |
200 |
TM100 |
6 |
|
C324-XX3X |
31.8 |
600 |
150 |
2543 |
70 |
200 |
TM100 |
3 |
|
C412-XX3X |
51.8 |
300 |
520 |
4245 |
122 |
500 |
TM140 |
4 |
|
C424-XX3X |
51.8 |
600 |
520 |
8817 |
244 |
500 |
TM140 |
2 |
General Clotho, Thalia, Atropos programming recommendations based on ID 1 mm lumen:
-
broth flow: ml/min/lumen/m – 15 - 25
-
harvest velocity: m/sec - 0.1 - 0.5
-
cleaning velocity: m/sec - 2 - 5
See much more explanations here - https://perfusecell.com/perfusion-support/tips-tricks/
Some abbreviations:
SUP = Single-Use-Pump = Thalia (or Clio)
SUB = Single-Use-Bioreactor = one of the sizes of CellVessel
HFF = Hollow-Fiber-Filter = SepraPor
ATF = Alternating-Tangential-Flow = method, principle
TFF = Tangential-Flow-Filtration = method, principle
CFF = Cross-Flow-Filter = broad designation
HFF Hold-Up volume:
- is the combined volume inside the multiple lumens
- not included volume inside the cartridge tube and outside of the lumen
See much more explanations here - https://perfusecell.com/perfusion-support/glossary