Which SUP / HFF volume ration insuring sufficient velocity during HFF cleaning
Which ratio between SepraPor XFCM20 and Thalia to select ? The entire P-SUB ATF portfolio follow this chart.
|
Brand |
OD mm |
total |
HFF |
HFF |
HFF Hold-Up |
Thalia stroke |
Selected SUP |
effective |
|
SepraPor |
diameter |
length |
Lumen |
surface |
volume |
volume |
Thalia |
ratio |
|
part # |
mm - inch |
mm - inch |
pcs / # |
cm2 |
cm3/ml |
cm3/ml |
model |
Thalia cm3 / HFF cm3 |
|
C008-XX1X |
9.5 - 3/8 |
200 - 8 |
13 |
75 |
2 |
15 |
TM60 |
7 |
|
C012-XX1X |
9.5 - 3/8 |
300 - 12 |
13 |
110 |
3 |
15 |
TM60 |
5 |
|
C024-XX1X |
9.5 - 3/8 |
600 - 24 |
13 |
220 |
7 |
50 |
TM80 |
7 |
|
CZ12-XX1X |
12.0 - 1/2 |
300 - 12 |
22 |
186 |
5 |
50 |
TM80 |
10 |
|
CZ24-XX1X |
12.0 - 1/2 |
600 - 24 |
22 |
406 |
12 |
50 |
TM80 |
4 |
|
C112-XX2X |
19.1 - 3/4 |
300 - 12 |
55 |
430 |
13 |
50 |
TM80 |
4 |
|
C124-XX2X |
19.1 - 3/4 |
600 - 24 |
55 |
932 |
26 |
200 |
TM100 |
8 |
|
C212-XX2X |
25.4 - 1 |
300 - 12 |
115 |
938 |
26 |
200 |
TM100 |
8 |
|
C224-XX2X |
25.4 - 1 |
600 - 24 |
115 |
1949 |
54 |
200 |
TM100 |
4 |
|
C312-XX3X |
31.8 - 1 1/4 |
300 - 12 |
150 |
1224 |
35 |
200 |
TM100 |
6 |
|
C324-XX3X |
31.8 - 1 1/4 |
600 - 24 |
150 |
2543 |
70 |
200 |
TM100 |
3 |
|
C412-XX3X |
51.8 - 2 |
300 - 12 |
520 |
4245 |
122 |
500 |
TM140 |
4 |
|
C424-XX3X |
51.8 - 2 |
600 - 24 |
520 |
8817 |
244 |
500 |
TM140 |
2 |
General Clotho, Thalia, Atropos programming recommendations based on ID 1 mm lumen:
-
broth flow: ml/min/lumen/m – 15 - 25
-
harvest velocity: m/sec - 0.1 - 0.5
-
cleaning velocity: m/sec - 2 - 5
See much more explanations here - https://perfusecell.com/perfusion-support/tips-tricks/
SepraPor basics part number details:
XFCM20 = 0.2 µm / 200 nm pore
XFCM40 = 0.4 µm / 400 nm pore
C112 = defines diameter and length in inches = OD 1 inch, length 12 inch
XX1X = defines connections where X position 2 and 3 is permeate barbs
Some abbreviations:
SUP = Single-Use-Pump = Thalia or Clio
SUB = Single-Use-Bioreactor = opposite of STR being a glass/steel Stirred-Tank-Reactor
HFF = Hollow-Fiber-Filter = SepraPor brand from Meissner Corp
ATF = Alternating-Tangential-Flow = method, principle
TFF = Tangential-Flow-Filtration = method, principle
CFF = Cross-Flow-Filter = broad designation
HFF Hold-Up volume:
- is the combined volume inside the multiple lumens
- not included volume inside the cartridge tube and outside of the lumen
See much more explanations here - https://perfusecell.com/perfusion-support/glossary