ATF-SUBmerged™ 250 kit for Modular mini SUBs
Revolutionizing CellRetention P-SUB-250 ATF kit integrating SepraPor® HFF (Hollow-Fibre-Filter) into the SUB eliminates dead volumes. Direct coupled HFF to inverted innovative LASER guided Thalia diaphragm Single-Use-Pump reduce shear stress, eliminate cell accumulation and well known HFF fouling.
CellRetention 250 ml P-SUBmerged unique arrangement integrates all 3 components in one unit for very easy Modular PCS installation. Modular takes care of agitation, aeration, pH, DO and media. Clotho Drive Unit controls Thalia which takes care of ATF perfusion and protects the HFF.
Clotho Drive Unit integrates pressures, vacuum, volume sensing eliminating the need for external sensors for HFF pressure drop, mass-flow measurements. Clotho process software masters accurately the programmable stroke volume, number of strokes, the allowed pressure drop along the HFF for fully automatic low broth harvest velocity and fully selectable higher broth velocity for the sequential lumen cleaning eliminating HFF blockage.
Left photo the Modular PCS with two SUBmerged-250 ATF kits. Center photo shows the Modular kit ready for installation. Right photo shows the 250 ml ATF P-SUB vacuum packed. Combine the "Easy-Connect" 250 ml SUB with our SUBmerged kit integrating 110 cm2 surface area HFF and TM60 SUP.
- The SUBmerged kit unify SepraPor™ 110 cm2, 0.2 µm HFF (manufactured by Meissner Corp) and on top of the HFF the inverted Thalia TM60 SUP which cannot trap cells or micro carriers
- The standard 250 ml SUB aeration is based on a simple tube with one 1 mm hole generation huge bubbles sufficient for batch operation. Summer 2026 the "CellRetention-250 ATF kit Modular" includes a 25 µm micro sparger body improving KLa values significant for high cell densities
- Thalia™ LASER guided Single-Use-Pump controlled by outstanding Clotho™ and its software
- Alternating broth volume and velocity fully programmable, accurately measured and fully repeatable – no process guessing
- SUBmerged™ and Direct-Fit HFF reduce shear stress caused by conventional Luer-Lok™ fittings
- The complete and pre-assembled SUBmerged kit is packed dry in dual film bags and precision irradiated – easy to install the kit under the hood
- Insert the SUBmerged kit in the 20 mm sampling port of the SUB, pre-wet the HFF and ready to use
SUBmerged 250 ATF HFF for Modular
Submerged Modular 250 kit
CellRetention ATF for Modular
Installation (inside the hood) is this simple - remove the original 20 mm sample port silicone plug, insert the SUBmerged kit into the sample port, add a drop of suitable liquid to the plug front as lubricant and push the kit in place - that’s it !! Install "Easy-Connect" 250 ml SUB in Modular and pre-wet HFF as usual.
All products are patented by www.stobbe.group