SUBmerged™ ATF P-SUB size 2 liter VV keep HFF free from fouling
Benefits of this particular P-SUBmerged are:
- avoid HFF fouling – freedom to select broth velocities for gentle harvest and sequential pulses flushing out debris from inside the HFF
- eliminate dead volume – skip all the hoses, traditional plastic connectors and SUBmerge the HFF
- avoid cell death - inverted Thalia pump arranged on top of the HFF = hereby the diaphragm pump cannot accumulate cells
- reduce shear force – protect cells by eliminating hoses, fittings - SUBmerge the HFF inside the P-SUB and the inverted Thalia on top of HFF
- increased Kla values - aeration not via small micro sparger - 30% better via large surface area tubular pico-sparger
Industry leading architecture P-SUB pre-installed ready for use:
- 500 – 1.5 liter Working Volume (WV) fully pre-assemmbled P-SUB (2 liter Vessel Volume OD 110 mm)
- Alternating broth flow by Thalia diaphragm Alternating-Single-Use-Pump (TM80 A-SUP)
- SepraPor® HFF (Hollow-Fibre-Filter) size 12x300, 170 cm2 surface SUBmerged with direct Thalia connection (no Luer-Lok or TRI-clamps) for reduced shear stress
- Inverted ground-breaking LASER guided Thalia TM60 diaphragm Single-Use-Pump (A-SUP)
- Single-Use-Sensors (SUS) for DO, pH, (optional InCyte bio-mass on SUB wall), temp, level as needed
CellRetention-2 ATF extended KLa
CellRetention-2 ATF tight headplate
CellRetention-2 ATF on HSF in MST
Photo show VisiWell and OneFerm sensors. All three photos show the 2 liter P-SUB and further mounted in the Heating-Support-Foot (HSF) bolted on the Magnetic-Stirrer-Table (MST) separating the black heavy servo motor from the P-SUB. The HSF is a perfect thermal control with sufficient contact to the SUB surface for accurate temperature regulation. Red LASER sensor mounted in a Re-Usable-Bracket above the dome of the inverted Thalia TM60 A-SUP. Clotho Drive Unit require the cable to the LASER sensor and the blue pneumatic hose for drive gas to Thalia.
General features of CellRetention™ medium size P-SUB:
- No space consuming heavy servo motor attached to the small diameter bioreactor head plate - take advantage of Magnetic-Bottom-Drive (MBD) of the P-SUB
- Designed with 5 x PG 13.5 head plate ports and 6 ports for In&Out with small diameter hoses, center port equipped with VisiWell for insert of VisiFerm DO.
- Thalia exchanged broth volume fully programmable from Clotho Drive Unit mastering accurately velocity, volume, number of variable strokes – no guessing
- SepraPor HFF manufactured by Meissner Corp with 170 cm2 area, Direct-Fit coupled, lower shear stress
- The complete and pre-assemmbled P-SUB unit packed in dual film bags and precision irradiated – forget the autoclave
- Thermal control with the Heating-Support-Foot (HSF) free to select either 110 VAC or 230 VAC
Further P-SUB medium features:
- Comes with Magnetic-Bottom-Drive (MBD) for up to 350 RPM used with MST
- One pitch blade impeller
- 3 small diameter weldable hoses length 900 mm
- 1 small short hose with 5 cm2 sterile filter for aeration
- 1 small short hose with swabable valve for bottom sampling
- Optional exhaust gas cooler
- 1 exhaust hose with 26 cm2 sterile filter - select the Sterile-Filter-Heater