SUBmerged™ P-SUB clear HFF from fouling
- Revolutionizing CellRetention-3 SUBmerge SepraPor® HFF (Hollow-Fiber-Filter) directly into the SUB broth eliminating dead volume
- Direct coupled HFF with inverted Thalia TM80 diaphragm Single-Use-Pump reduces shear stress, eliminates cell accumulation and clear well-known HFF fouling
SUBmerged 3 MBD+C112+TM80 Straight
SUBmerged-3 TM80+SepraPor C112
SUBmerged-3 in HSF+motor+Incyte
The re-invented 3.2 liter P-SUB with SUBmerged SepraPor C112 and inverted Thalia TM80S on top. The InCyte biomass SUS patch mounted on the vessel wall and InCyte ARC sensor clicked on. 3.2 liter P-SUB mounted in the Heating-Support-Foot (HSF) on the Magnetic-Stirrer-Table (MST). Reason is simple – not space for both servo motor, sensors and Thalia TM80S on the OD137 mm head plate so Magnetic-Bottom-Drive (MBD) is the smart option.
Inside the P-SUB
- Agitation by OD 60 mm triple pitch blade impeller, super-efficient 25 or 100 µm pore glass micro sparger for high cell density, deep-tubes, addition, harvest, baffles, etc – see doc at download
- SepraPor XFCM20C112-xx1x HFF (Hollow-Fibre-Filter) with 420 cm2 surface and HFF inlet SUBmerged directly into the broth
- Single-Use-Sensors (SUS) for DO, pH, bio-mass, temp, level as needed
Outside the P-SUB
- Thalia diaphragm Alternating-Single-Use-Pump (A-SUP) straight or angled version interfaced with the SepraPor HFF – no TRI-clamps
- MBD (Magnetic-Bottom-Drive) or HPD (Head-Plate-Drive) as needed
- 900 mm weldable M-Flex hose connected to SepraPor permeate barb
- 900 mm weldable M-Flex hose connected to unique 25 or 100 µm pore glass frit
- Thermal control as needed - electrical or liquid with Magnetic-Bottom-Drive (MBD) or Head-Plate-Drive (HPD) - choose one of these options:
- Electrical-Heating-Blankets (EHB) (InCyte possible - require EHB attachments)
- Electrical via Heating-Support-Foot (HSF) (InCyte possible) - see the 3rd photo
- Liquid RUJ - P-SUBmerged to be inserted into the Re-Useable-Jacket (RUJ) (InCyte not possible)
- Liquid SUJ - equipped with Single-Use-Jacket (SUJ) (InCyte not possible)
The kit / the package consists of:
- 0.4 - 2.5 liter Working Volume (WV) from 3.2 liter Vessel Volume (VV) (dim OD137 x 245 mm) fully pre-assembledand ready to install with known impeller Power Numbers
- The CellRetention-3 dual foil bag packed and precision irradiated
- Don’t forget to pre-wet HFF as usual – info found here – Tips&Tricks
Benefits of the P-SUBmerged are:
- avoid HFF fouling– freedom to select broth velocities for gentle harvest and sequential pulses flushing out debris from inside the HFF
- eliminate dead volume– skip all the hoses, traditional plastic connectors and SUBmerge the HFF
- avoid cell death- inverted Thalia pump arranged on top of the HFF = hereby the diaphragm pump cannot accumulate cells
- reduce shear force– protect cells by eliminating hoses, fittings - SUBmerge the HFF inside the P-SUB and the inverted Thalia on top of HFF
Installation
- Suitable for a number of PCS (Process-Control-Systems) with variation in hose / peri pumps connections, agitation – check under download the standard variant currently available
- A number of servo motor adaptors are available
CellRetention 3 P-SUB ATF HSF MST
CellRetention 3 P-SUB+ATF+HSF+MST
CellRetention-3 B-SUB SUJ
Rendered SolidWorks CAD drawings show different details of the 3 liter P-SUBmerged all 3 driven from bottom. Left P-SUB arranged in the Heating-Support-Foot (HSF) on MST incl InCyte opposite. Center - the 3 liter P-SUB surrounded by the Single-Use-Jacket (SUJ) for liquid thermal control. Right - the P-SUB incl SUJ illustrating how liquid thermal control require liquid inlet at bottom and outlet opposite at top.
Clotho Drive Unit is required and integrates pressures, vacuum, volume sensing eliminating the need for external sensors for HFF DeltaP / ∆P pressure drop, mass-flow measurements. Clotho process software controls accurately the programmable stroke volume, number of strokes, the allowed pressure drop along the HFF for fully automatic broth low harvest velocity and fully selectable broth high velocity for the sequential lumen cleaning eliminating HFF blockage.
SUBmerged 3 SepraPor+Angled Thalia
RUJ for 3 liter P-SUB Stainless Steel HPD
OD137 P-SUB with EHB 75x350
If Head-Plate-Drive (HPD) is the desired option – not the safest option for a lightweight P-SUB with a heavy servo motor on top – though if the P-SUBmerged require liquid thermal control balance is obtained. If the P-SUB is inserted into the Re-Useable-Jacket (RUJ) safety is obtained. The P-SUB mounted in a 15 mm deep mechanical support on MST and wrapped with the 75 x 350 mm EHB.
Find Tips & Tricks here - https://perfusecell.com/perfusion-support/tips-tricks-in-details/tips-tricks-3-liter/
FlowChart connects the P-SUBmerged to the more advanced Cronus-PCS running Lucullus SCADA software. Rendered CAD illustrate the 3 liter SUBmerged with straight Thalia for MST drive and with angled Thalia TM80A for HPD drive. Both designed for HSF thermal control allowing InCyte to be installed. Right photo shows latest sparger concept with multiple micro porous glass frits for high KLa numbers arranged under the impeller.
Below find a complete set of product documentation
The one 3D PDF file below needs to be download and a correction to Acrobat is needed - https://perfusecell.com/download-product-info
