0.4 - 2 liter Working Volume TFF Perfusion-SUB

P-SUB integrating diaphragm pump, HFF and sensors

World only fully assembled P-SUB for TFF method offer:

  • SepraPor HFF (Hollow-Fibre-Filter) for fouling free process integrated with innovative LASER guided diaphragm pump
  • 0.4 - 2 liter Working Volume (WV) fully pre-assembled P-SUB - not in lab extra assembling in the hoof needed
  • Broth circulated by Clio diaphragm One-way-Single-Use-Pump (O-SUP) for gentle harvest and seqouential instant HFF cleaning pulse 

Rendered assembly of integrated SUB with Clio CM80 O-SUP and 300 mm long SepraPor HFF. Fully pre-assembled, packed, E-beam irradiated and ready to use as Perfusion-Single-Use-Bioreactor. Flow-chart illustrates most important connections between CellMembra and Cronus Tower.

CellMembra-3-MST integrates SUB / O-SUP / HFF / SUS in one package

  • 3.2 liter Vessel Volume (VV) with 5 x PC 13.5 ports
  • 0.4 – 2 liter Working Volume (WV)
  • Integrate OneFerm pH and VisiWell or PolarWell and InCyte patch by Hamilton
  • 2 free PG 13.5 port for other sensors
  • O-SUP is LASER guided Clio CM80 featuring measured broth volume and velocity by Clotho Drive Unit

HFF - pre-installed

  • Customized SepraPor HFF (Meissner Corp) cartridges with 0.2 µm pore, 1.0 mm straw/lumen, 420 or 850 cm2 area, integrated into Clio O-SUP, integrated barb retentate for low stress return to SUB, NO TRI-clamps needed 

CellMembra-3 with Clio CM80 integrating SepraPor C112 mounted in HSF on MST. The InCyte biomass sensor seen attached to vessel wall mounted patch. Cronus Tower for full control.

Features for the CellMembra 3 litre P-SUB

  • Fully assembled P-SUB packed in dual film bags and precision E-beam irradiated - ready to use
  • Three baffles of width 10 mm x thickness 4 mm – if you have other requirements – tell us
  • Impeller: Kidney impeller, OD 60 mm, 3 blades, 30 degrees clock wise rotation and up flow mounted 50 mm from the bottom – if you have other requirements – tell us
  • Pumped volume and velocity measured accurately and fully controlled – no guessing

More information about SepraPor HFF under Support