SUBmerged™ mini Perfusion-SUB
- Revolutionizing CellRetention-300-DASbox SUBmerge SepraPor® HFF (Hollow-Fibre-Filter) directly into the SUB broth eliminating dead volume – even without occupying a precious PG13 port !!!!
- HFF direct coupled to inverted innovative LASER guided Thalia diaphragm Single-Use-Pump reduce shear stress, eliminates cell accumulation and clear the well known HFF fouling
CellRetention-300-ATF BioBLU
dual BioBLU batch and perfusion
DASbox with BioBlu ATF and Clotho
Photo shows standard bioBLU 0.3 batch SUB converted to CellRetention-300-DASbox operating ATF P-SUBmerged. Center photo one std bioBLU® batch and one CellRetention-300 with the blue head plate and visible the Thalia TM60 on top of SUBmerged SepraPor®. Right photo batch, ATF SUBs and Clotho Drive Unit and LASER sensor in front of DASbox dummy.
INSIDE the CellRetention-300-DASbox:
- SepraPor® HFF C012 inlet positioned directly in the broth (manufactured by Meissner® Corp) with pore 0.2 µm, 110 cm2 area, 1 mm lumen
- Two liquid level sensors
OUTSIDE the CellRetention-300-DASbox:
- Based on customized BioBLU®3 batch SUB designed with magnetic HPD for the DASbox. Aeration, agitation, and process control remain based on the standard BioBLU® 0.3c and chosen DASbox® configuration.
- LASER guided Thalia inverted SUP eliminates cell accumulation – choose between TM60 or TM80
- Hoses in-mass covering any need
- Included OneFerm pH sensor with K8 connection mounted in PG13 port
- One PG13 port free
The P-SUB features:
- The complete and pre-assembled mini-P-SUB packed in dual film bags and precision irradiated – forget the autoclave
- P-SUB dim OD76 x 120 mm for Working Volume (WV) ranging from 80 ml up to 200 ml
- Part of DASbox is the MP8 peristaltic pump module - the integrated Pharmed/Biomed pump hoses are selectable ID 1.0 (media exchange) or 0.5 (addition for pH control)
- Thermal control, agitation, aeration, media addition as offered by DASbox
Benefits:
- Clotho drive unit offer controlled alternating broth volume, number of strokes and velocity fully programmable, accurately measured and fully repeatable – no process guessing check out Clotho here - https://perfusecell.com/perfusion-bioreactors/diaphragm-pump-drive-units-41
- SUBmerged™ and Direct-Fit™ HFF eliminating shear stress caused by conventional Luer-Lok™ fittings
- avoid HFF fouling – freedom to select broth velocities for gentle harvest and sequential pulses flushing out debris from inside the HFF
- eliminate dead volume– skip all the hoses, traditional plastic connectors and SUBmerge the HFF
- avoid cell death - inverted Thalia pump arranged on top of the HFF = hereby the diaphragm pump cannot accumulate cells
- reduce shear force – protect cells by eliminating hoses, fittings - SUBmerged HFF inside the P-SUB and the inverted Thalia on top of HFF
Installation (no hood needed):
- Unpack and insert the CellRetention-300-DASbox in DASbox
- Connect all the hoses and sensors – being a big job !
- Clamp in the LASER sensor in the red Thalia socket and connect to Clotho
- Don’t forget to pre-wet HFF, as usual, by adding 200 ml suitable liquid into the SUB. Activate Clotho for 15 strokes and pump entire volume out through the permeate port by MP8 peri pump
Find Tips & Tricks here - https://perfusecell.com/perfusion-support/tips-tricks-in-details/tips-tricks-small-volume
Photo show bioBLU 0.3 batch SUB converted to CellRetention-300-DASbox operating ATF P-SUBmerged. Right photo four CellRetention-300 with the blue head plate and visible the Thalia TM80 on top of SUBmerged SepraPor® and one bioBLU® 1 liter equipped with the PG13-DIY-kit.
SUBmerged 0.3 ATF HFF DASbox
CellRetention-300-DASbox ver1
CellRetention-300-DASbox supplied
Flow-chart illustrates most important connections. Rendered JPG illustrate extensive set of hoses that fit directly to DASbox and as such require no assembling inside a hood. Photo of the complete assembly as delivered – ready to install - remember to wet/rinse the HFF before use.
Some conclusions for the CellRetention-300-DASbox:
- The oxygenation is based on the BioBLU 0.3 standard one hole gas inlet limiting the max suspended cell density ranging 50-75 m/cells/ml
All products are patented by www.stobbe.group
