SUBmerged™ ATF P-SUB keep the HFF clean

  • Revolutionizing CellRetention-13 SUBmerge SepraPor® HFF (Hollow-Fiber-Filter) directly into the SUB broth eliminating dead volume
  • Direct coupled HFF with inverted Thalia TM100 diaphragm Single-Use-Pump reduces shear stress, eliminates cell accumulation and clear well-known HFF fouling
SUBmerged SepraPor HFF + Thalia
BIG

SUBmerged SepraPor HFF + Thalia

SUBmerged 13 with motor HPD
BIG

SUBmerged 13 with motor HPD

SUBmerged 3 and 13 liter
BIG

SUBmerged 3 and 13 liter

The SUBmerged OD200 mm 13 liter P-SUP with Thalia TM100 mounted inverted on the SepraPor C224 HFF. The red LASER sensor in real-time measure the position of the diaphragm for unique operation. Hereby full control of velocity and selected exchange volume of broth. Pre-assembled P-SUBmerged 3 and 13 with InCyte bio-mass, VisiFerm and Oneferm SUS.   

INSIDE the P-SUB

  • SepraPor HFF model XFC20C224-xx2x 600 mm long with 1,500 cm2 surface
  • dual OD100 mm pitch blade impellers
  • 4 baffles
  • Single-Use-Sensors (SUS)

OUTSIDE the P-SUB

  • Headplate drive for almost any servo motor
  • Variety of hoses
  • Check out specification page / doc under download

The kit / the package consists of:

13 liter Vessel Volume (VV) - Working Volume (WV) as low as 2-4 liter in a rigid OD200 x 445 mm PolyCarbonate vessel

  • SUBmerged SepraPor C224-xx2x HFF in a fouling free operation unifying with innovative LASER guided Thalia diaphragm pump
  • The kit as standard integrates TM100 with a dry stored 0.2 µm pore size SepraPor HFF model C224-xx2x 600 mm long with 1,500 cm2 surface area. Alternatively 2,300 cm2 filter surface area from C324-xx3x.
  • Alternating broth by Thalia-M100 (or TM140) diaphragm Alternating-Single-Use-Pump (A-SUP)

Thalia TM100 A-SUP = Alternating-Single-Use-Pump

  • The diaphragm Alternating-Single-Use-Pump (A-SUP) exchange back and forth broth from inside the SUB through the HFF at selected velocities - mainly low for harvest and sequentially few high for cleaning
  • The high precision LASER sensor measures in real-time accurately the position of the diaphragm so broth velocity and exchanged volume can be calculated
  • Thalia TM100 (or the TM140) will exchange any volume within its spec programmable from 0.1 to >10 m/sec velocity through the HFF securing the HFF stays clean throughout the entire process

Single-Use-Sensors (SUS) to choose from:

  • SUBmerged CellRetention™ features pre-installed 420 mm long DO VisiFerm and OneFerm pH (either K8 or VP)
  • level/ground as standard
  • Available with InCyte bio-mass SUS patch mounted horizontal on the wall of the 13 liter P-SUB vessel

Benefits of the P-SUBmerged are:

  • avoid HFF fouling– freedom to select broth velocities for gentle harvest and sequential pulses flushing out debris from inside the HFF
  • eliminate dead volume– skip all the hoses, plastic connectors and SUBmerge the HFF
  • avoid cell death- inverted Thalia pump arranged on top of the HFF = hereby the diaphragm pump cannot accumulate cells
  • Reduce shear force stress – eliminate hoses, traditional fittings and SUBmerge the HFF directly into the broth inside the P-SUB

Installation

2 - 10 liter Working Volume (WV) fully pre-assembled, dual foil bagged and E-beam irradiated ready to use

Thermal Control

  • Electrical-Heating-Blanket (EHB) - wrap the orange silicone EHB around the P-SUB
  • Liquid Re-Usable-Jacket (RUJ) for heating/cooling with HPD drive 
  • Liquid Single-Use-Jacket (SUJ) integrating the P-SUB inside the SUJ

Agitation:

  • via Head-Plate-Drive (HPD) directly by available servo motor and the appropriate adaptor
  • Via Magnetic-Bottom-Drive (MBD) driven by the Magnetic-Stirrer-Table (MST)
  • dual OD100 mm pitch blade impellers - find Power Numbers here

Drive units:

  • CellRetention™ P-SUB and Clotho or Lachesis works with most PCS over OPC-UA and software like Lucullus and DeltaV
  • Drive Units Clotho and Lachesis designed to master the scalable Thalia A-SUP operation and ensure efficient and reproducible operation
  • The A-SUP Drive Units feature programmable broth/retentate velocity and volume through the HFF module for gentle harvest and sequential fast removal of HFF membrane deposits
  • Thalia (depending on SUP size) operated by either Clotho or Lachesis Drive Unit 
  • Drive unit connection to pressurized air and vacuum within the lab

Don’t forget to pre-wet HFF as usual – info found here – Tips & Tricks

SUBmerged ATF HFF Cronus
BIG

SUBmerged ATF HFF Cronus

From left FlowChart on SUB and the InCyte ARC modules attached. Center shows some of the 600 different impellers to choose from. Left an example of a SUB with an extensive assembly of hoses and bottles.

All products are patented by www.stobbe.group

 

© 2026 PerfuseCell-Cronus A/S