Integrated mini Perfusion-SUB
- Revolutionizing CellMembra-300-DASbox integrates Clio SUP (Single-Use-Pump) with SepraPor® HFF (Hollow-Fibre-Filter) directly into the SUB broth reducing dead volume – even without occupying a precious PG13 port !!!!
- Direct coupled HFF to innovative LASER guided Clio diaphragm SUP reduce shear stress and clear well known HFF fouling
CellMembra-300 + Clio CM80
CellMembra-300 TFF Clio+SepraPor
CellMembra-300 Clio+SepraPor detail
BioBLU 0.3 SUB converted to CellMembra-300-DASbox P-SUB. Center CellMembra-300 (ver-10) with the blue head plate and visible the Clio CM80 SUP. Right CellMembra-300-DASbox illustrating the in SUP integrated SepraPor HFF eliminating Luer-Loc hereby reducing significant shear force stress.
INSIDE the P-SUB the CellMembra-300-DASbox integrates:
- SepraPor® HFF C012 cartridge (manufactured by Meissner® Corp) with pore 0.2 µm, 110 cm2 area, 1 mm lumen inlet integrated directly on Clio OneWay-Single-Use-Pump (O-SUP)
- Two liquid level sensors
- Extra deep-tube as SUP inlet and HFF return
OUTSIDE the P-SUB the CellMembra-300-DASbox integrates:
- Based on customized BioBLU®3 batch SUB designed with magnetic HPD for the DASbox. Aeration, agitation, and process control remain based on the standard BioBLU® 0.3c and chosen DASbox® configuration.
- LASER guided Clio TM80 SUP position eliminates dead volume
- Hoses in-mass covering any need
- OneFerm pH sensor with K8 connection included mounted in PG13 port
- One PG13 port free
The P-SUB ver-10 features:
- The complete and pre-assembled mini-P-SUB packed in dual film bags and precision irradiated – forget the autoclave
- P-SUB dim OD76 x 120 mm for Working Volume (WV) ranging from 80 ml up to 200 ml
- Part of DASbox is the MP8 peristaltic pump module - the integrated Pharmed/Biomed pump hoses are selectable ID 1.0 (media exchange) or 0.5 (addition for pH control)
- Thermal control, agitation, aeration, media addition as offered by DASbox
Benefits:
- Clotho drive unit offer controlled tangential flow of any broth volume, number of strokes and velocity fully programmable, accurately measured and fully repeatable – no process guessing – check out Clotho here - https://perfusecell.com/perfusion-bioreactors/diaphragm-pump-drive-units-41
- SUBmerged™ and Direct-Fit™ HFF eliminating shear stress caused by conventional Luer-Lok™ fittings
Installation (no hood needed)
- Unpack and insert the CellMembra-300-DASboxin DASbox
- Connect all the hoses and sensors – being a big job !
- Clamp in the LASER sensor in the red Clio socket and connect to Clotho
- Don’t forget to pre-wet HFF, as usual, by adding 200 ml suitable liquid into the SUB. Activate Clotho for 15 strokes and pump entire volume out through the permeate port by MP8 peri pump
Find Tips & Tricks here - https://perfusecell.com/perfusion-support/tips-tricks-in-details/tips-tricks-small-volume
CellMembra-300-DASbox supplied
DASbox with two CellMembra TFF
CellMembra triple BioBlu ver-8
The CellMembra-300-DASbox is delivered dual bag packed and E-beam irradiated – ready to install - remember to wet/rinse the HFF before use. Heavily equipped with an extensive hose assembly ready to install and use in the DASbox. Example of how CellMembra-300 P-SUBs in parallel inserted into the DASbox originally designed for OD76 mm STR.
P-SUB 300 TFF + HFF + DASbox
CellMembra-300 dual Clotho towers
CellMembra-300 ver-10 for Merck
Flow-Chart illustrates the setup with Clotho Tower (in the yellow box) operating the LASER guided Clio SUP. Clotho keep an eye on DeltaP / ∆P and ensures regular cleaning of the HFF. Center photo 8 pcs P-SUBs in parallel, operated, controlled by dual Clotho towers and DASware or directly from the Clotho software independent of DASware. The pressurized air and vacuum delivered by dual Alagonia and one Sarpedon for each tower. A photo of a CellMembra-300 Clio SUP lot from production.
The below one 3D PDF file needs to be download and perhaps a correction to Acrobat is needed - https://perfusecell.com/download-product-info
