PerfuseCell offer pre-assembling P-SUBs including HFF supplied by:
- Meissner (SepraPor brand) available in various cartridge diameters, length 300 mm and 600 mm or more – hereby surface area selectable, customized cartridges pre-installed combined with from 0.25 up to 30 liter SUBs and SUPs, without Luer-Loc or TRI-clamps, creating a complete unique P-SUB range of products
- SepraPor, Polyethersulfone (PES), pore size 200 nm, dry storage, 1 mm lumen inner diameter
- SepraPor, Polyethersulfone (PES), pore size 10 kD, Glycerine wetted storage, 1 mm lumen inner diameter, pre-installed in wetted stage, washed-in-place creating a complete and unique P-SUB product
Chart of Meissner smaller HFF portfolio - Meissner’s examples of 300 mm long HFF with TRI clamps.
- Repligen (Spectrum brand), Polyethersulfone (PES) material, model P20U, dry storage, pore size 0.2 µm (200 nm), 1 mm fiber diameter, standard lengths, PES modules pre-installed ready for use - just unpack the complete P-SUB from the dual bag enclosure
- Note the Repligen model D02 material mPES have 12 fibers and D02 material PES have 14 fibers – influence on surface area and performance calculations!
Chart of Repligen smaller HFF - Selection of HFFs ranging 100 - 900 mm length.
General recommendations
- Circulate DI water, buffer or media after unpacking the P-SUB and check permeate reach 2 ml/cm2 flow rate
- Follow manufactures recommendation is always good!
- See also our Trips & Tricks
Left – CellMembra BioBLU 0.3 P-SUB TFF integrating the horizontal arranged Clio CM80 O-SUP integrating the HFF cartridge without restricting Luer-Lok - ready for use. Center – 250 ml Modular ATF and 3.2 liter ATF. Right – Thalia TM140 A-SUP mounted with a SepraPor HFF with 1½” TRI-flange and the small Thalia M80 with ½” TRI SepraPor.
Choose HFF from one of your preferred suppliers, such as:
- HFF from Meissner - www.meissner.com/products/tangential-flow-filters-seprapor
- HFF from Repligen - www.repligen.com/technologies/spectrum-filters
- HFF from Cytiva - www.cytivalifesciences.com/en/us/shop/bioprocessing-filtration/tangential-flow-filtration/hollow-fiber-cartridges
