SUBmerged™ ATF P-SUB keep the HFF free from fouling
This unique 3.2 liter bioreactor operate as Perfusion-SUBmerged (P-SUB) after the CellRetention ATF method.
Benefits of the P-SUBmerged are:
- avoid HFF fouling – freedom to select broth velocities for gentle harvest and sequential pulses flushing out debris from inside the HFF
- eliminate dead volume – skip all the hoses, traditional plastic connectors and SUBmerge the HFF
- avoid cell death - inverted Thalia pump arranged on top of the HFF = hereby the diaphragm pump cannot accumulate cells
- reduce shear force – protect cells by eliminating hoses, fittings - SUBmerge the HFF inside the P-SUB and the inverted Thalia on top of HFF
SUBmerged 3 MBD+C112+TM80 Straight
SUBmerged 3 TM80+SepraPor C112
SUBmerged 3 in HSF+motor+InCyte
Left photo - The re-invented 3 liter P-SUB with SUBmerged SepraPor C112 and inverted Thalia TM80 with red LASER support on top. Centre photo - The InCyte biomass SUS patch mounted on the vessel wall and InCyte ARC sensor clicked on. Right photo - 3 liter P-SUB is mounted in the Heating-Support-Foot (HSF) on the Magnetic-Stirrer-Table (MST). Reason is simple – there is not space for both servo motor, sensors and Thalia TM80 on the OD137 mm head plate so Magnetic-Bottom-Drive (MBD) required.
P-SUBmerged 3.2 liter Vessel Volume features:
- 0.4 - 2.5 liter Working Volume (WV) fully pre-assembled and ready for use with selectable impellers of which Power Numbers are known
- Thalia diaphragm Alternating-Single-Use-Pump (A-SUP) straight or angled version integrating a
- SepraPor C112-xx1x HFF (Hollow-Fibre-Filter) with 420 cm2 surface SUBmerged directly into the broth
- Single-Use-Sensors (SUS) for DO, pH, bio-mass, temp well, level as needed
CellRetention 3 P-SUB ATF HSF MST
CellRetention 3 P-SUB+ATF+HSF+MST
CellRetention-3 B-SUB SUJ
Rendered SolidWorks CAD drawings show different details of the 3 liter P-SUBmerged all 3 driven from bottom. Left P-SUB arranged in the Heating-Support-Foot on MST incl InCyte opposite. Center - the 3 liter P-SUB surrounded by the Single-Use-Jacket (SUJ) for liquid thermal control. Right - the P-SUB incl SUJ illustrating how liquid thermal control require liquid inlet at bottom and outlet opposite at top.
Thermal control as needed - electrical or liquid with Magnetic-Bottom-Drive (MBD) or Head-Plate-Drive (HPD) - choose one of these options:
- Electrical-Heating-Blankets (EHB) (InCyte possible - require EHB attachments do not interfere)
- Electrical via Heating-Support-Foot (HSF) (InCyte possible) - see the 3rd photo
- Liquid RUJ - P-SUBmerged to be inserted into the Re-Useable-Jacket (RUJ) (InCyte not possible)
- Liquid SUJ - equipped with Single-Use-Jacket (SUJ) (InCyte not possible)
SUBmerged 3 SepraPor+Angled Thalia
RUJ for 3 liter P-SUB Stainless Steel HPD
OD137 P-SUB with EHB 75x350
If Head-Plate-Drive (HPD) is the desired option – not the safest option for a lightweight P-SUB with a heavy servo motor on top – though if the P-SUBmerged require liquid thermal control balance is obtained. If the P-SUB is inserted into the Re-Useable-Jacket (RUJ) safety is obtained. The P-SUB mounted in a 15 mm deep mechanical support on MST and wrapped with the 75 x 350 mm EHB.
SUBmerged 3 liter ATF HFF Cronus
CellRetention-3 P-SUB Thalia Straight
SUBmerged SepraPor Thalia Angled
FlowChart connects the P-SUBmerged to the more advanced Cronus-PCS running Lucullus SCADA software. Rendered CAD illustrate the 3 liter SUBmerged with straight Thalia for MST drive and with angled Thalia for HPD drive. Both designed for HSF thermal control allowing InCyte is installed.
All products are patented by www.stobbe.group