Tips & tricks we have picked up during our work with the 3 liter P-SUB

On this page randomly organised with Tips & Tricks for

• The Drive Unit Clotho mechanically and electronically 
• Software for the Drive Units 
• P-SUB's being CellMembra-3 and CellRetention-3
• Vessel Volume 3200 ml - Working Volume depending on a variety of parameters

We strongly recommend read various User Manuals

Remember that mammalian cells line behaves different to different conditions. Some cells do not like alternating flow, and some does. Some cells don't like re-circulating flow, and some does.

Variables for CellMembra-3 TFF-Integrating Clio-100

• Circulated volume, SP, Set Point: 0 – 1,300 ml/min
• Stroke volume, 100 % stroke/systole, ml: ~100
• Number of strokes/min: SP / 100 + C cycle time
• Cleaning / Harvest cycles, C/H ratio: 1 – 25
• Cleaning Velocity, max CV, m/sec: ~10
• C cycle time, ms: 0.05-0.1
• Harvest Velocity, max HV, m/sec: 0 – 1.5 depending on SP