Which SUP volume to insure sufficient velocity during HFF cleaning
Which ratio between SepraPor and Thalia to select ? The entire P-SUB ATF portfolio follow this chart.
|
|
OD mm |
total |
total |
HFF |
HFF |
Thalia stroke |
suggested |
effective |
|
SepraPor |
diameter |
length |
length |
surface |
hold-up |
volume |
Thalia |
ratio |
|
part # |
mm |
mm |
mm |
cm2 |
cm3 |
cm3 |
model |
? / ? |
|
C012-XX1X |
9.5 |
300 |
|
106 |
3 |
20 |
TM60 |
7 |
|
C024-XX1X |
9.5 |
|
600 |
220 |
6 |
20 |
TM60 |
3 |
|
|
12.0 |
300 |
|
230 |
6 |
20 |
TM60 |
3 |
|
C112-XX2X |
19.1 |
300 |
|
430 |
13 |
50 |
TM80 |
4 |
|
C124-XX2X |
19.1 |
|
600 |
932 |
26 |
50 |
TM80 |
2 |
|
C212-XX2X |
25.4 |
300 |
|
938 |
26 |
50 |
TM80 |
2 |
|
C224-XX2X |
25.4 |
|
600 |
1949 |
54 |
200 |
TM100 |
4 |
|
C312-XX3X |
31.8 |
300 |
|
1224 |
35 |
200 |
TM100 |
6 |
|
C324-XX3X |
31.8 |
|
600 |
2543 |
70 |
200 |
TM100 |
3 |
|
C412-XX3X |
51.8 |
300 |
|
4245 |
122 |
500 |
TM140 |
4 |
|
C424-XX3X |
51.8 |
|
600 |
8817 |
244 |
500 |
TM140 |
2 |
General Clotho, Thalia, Atropos programming recommendations:
-
flow per lumen: ml/min/lumen/m – 15 - 25
-
harvest velocity: m/sec - 0.1 - 0.5
-
cleaning velocity: m/sec - 2 - 5
See much more explanations here - https://perfusecell.com/perfusion-support/tips-tricks/
Some abbreviations:
SUP = Single-Use-Pump = Thalia
SUB = Single-Use-Bioreactor
HFF = Hollow-Fiber-Filter = SepraPor
ATF = Alternating-Tangential-Flow
TFF = Tangential-Flow-Filtration
CFF = Cross-Flow-Filter
See much more explanations here - https://perfusecell.com/perfusion-support/glossary