Clio™ keep the HFF clean as to sequential selectable fluid velocities
Clio is a fluid conveying OneWay-Single-Use-Pump (O-SUP) and part of CellMembra TFF-Integrated perfusion concept. Build for super accurate operation by the advanced Clotho or Lachesis Drive Units.
Clotho measures via the LASER accurately in real-time the free-flowing diaphragm position. Allowing software to decide volume to be convey and at desired fluid velocity. The result is an Air-Operated-Diaphragm-Positive-Displacement (AODPD) OneWay-Single-Use-Pump (O-SUP) with un-parallel performance.
Scalable - YES - check out spec table
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Clio O-SUP models |
CM50 |
CM80 |
CM100 |
CM140 |
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Availability |
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Diaphragm diameter, mm |
50 |
80 |
100 |
140 |
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Stroke Volume, SV, mL/S, 100% stroke |
30 |
60 |
200 |
500 |
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Cardiac Output (CO range), harvest cycle, max mL/min |
200 |
450 |
1,500 |
2,500 |
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Beats-per-Minute, BpM |
0-20 |
0-18 |
0-15 |
0-10 |
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Conditions |
P-SUB integrated |
P-SUB integrated |
P-SUB integrated |
P-SUB integrated |
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Drive Unit |
Clotho |
Clotho |
Clotho |
Lachesis |
Clio technical / functionality
- The diaphragm OneWay-Single-Use-Pump (O-SUP) integrates two passive OneWay valves guiding the direction of fluid. The valve body receives broth from the P-SUB and deliver broth to the Hollow-Fiber-Filter (HFF) feed inlet after which the broth passes axially the HFF and exit as retentate to be returned to the P-SUB
- Clio O-SUP integrates between the P-SUB and the HFF
- Clio features the re-usable (red) LASER sensor reading diaphragm position through the transparent dome window = know the position = optimum position programming
- As the position of the diaphragm is known at any time the broth velocity and volume calculation can be performed, repeated and presented on the Clotho display in real-time or by Lucullus
- Clio both pump and measures conveyed volume ranging within its spec and from 0 to >10 m/s velocity
Left photo show Clio Integrated with BioBlu 0.3 with red LASER sensor support. Center photo CM80 mounted Integrated on vessel side – NO TC or Luer-Loc - HFF Integrated on Clio. Right photo show CellMembra TFF-Integrated chart.
Unique user features:
- Clio keep the HFF - Hollow-Fibre-Filter - clean from fouling issues maximizes process time
- Clio handle fluids = liquids and / or gases or any mix of liquid/gas
- Clio is a true metering pump = Positive Displacement (PD) pump = mass-flow measured accurately and constantly
- Clio pump do NOT need a mass-flow sensor measuring the mass-flow
- Clio easily sucks fluids from low arranged containers
- Clio is a low shear force, gentle design which do not damage mammalian cells
- Clio pump do NOT need calibration like peristaltic pumps - Clio is self calibrating via intelligent software in Clotho, Lachesis
- Clio pump do NOT loose nano size silicone particles to the fluid like peristaltic pumps
The basics in operation is copied from the human heart functionality
- Cardiac Output (CO) = total pumped volume, SUP capacity, mL/min (CO = BpM x SV)
- Beats-per-Minute (BpM) = ranging 0 - 20 BpM, SUP diameter/stroke volume depending
- Stroke Volume (SV) = programable from 0 - 100% of SUP capacity
Clio Single-Use-Pump chamber are manufactured from medical grade Nylon. The diaphragm is manufactured from medical grade silicone. The two valves are manufactured from medical grade silicone. Platinum cured silicone or M-Flex in the dimension and length you may desire.
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