0.4 – 10 liter Working Volume with Thalia A-SUP
Traditional ATF-SUB pre-assembled in size 3 - 6 - 13 liter Vessel Volume CellRetention ATF-Traditional. P-SUB vessel size OD 137 or OD 200 mm combined with the Thalia TM80 or the TM140 A-SUP and Hollow-Fiber-Filter with TC clamps and SUS.
From left: Illustration shows fluid diagram with Magnetic-Bottom-Drive SUB, controlled diaphragm A-SUP and passive HFF. The customizable SUB suction deep-tube connects via a hose to the HFF top and at HFF bottom to the A-SUP. The A-SUP is mounted on a foot, and the red Laser sensor for real-time measuring the position of the diaphragm and hereby full control of velocity and selected exchange volume.
P-SUB 3 liter Ext + Thalia TM80
Thalia TM100 for P-SUB-6 Ext
CellRetention-3 ATF Ext incl assembly
From left: Pre-assembled P-SUB, A-SUP, HFF. The pre-assembled A-SUP/HFF is mounted on a foot. The red Laser sensor for real-time measuring the position of the diaphragm attached to the A-SUP. The Magnetic-Stirrer-Table slightly visible. Right photo – an extensive P-SUB with huge assembly and Thalia-100 A-SUP with ¾” TRI connection to the HFF.
P-SUB - for cultivation (the SUB is sourced from sister company CerCell):
- 100% configurable design for the 13 liter Vessel Volume (VV) - Working Volume (WV) as low as 2-3 liter
- Agitation from bottom by Magnetic-Bottom-Drive (MBD) driven by the Magnetic-Stirrer-Table (MST)
- Agitation from top, Head-Plate-Drive (HPD) and any type and number of impellers as an option
P-SUB - Thermal Control
- Electrical blanket heating - wrap orange silicone blanket around
- Electrical Jacket heating combined with with Magnetic-Bottom-Drive - metal foot also for stability
- Water Re-Usable-Jacket (RUJ) for heating/cooling even combined with Magnetic-Bottom-Drive (MST)
HFF – harvest continuous a cell free permeate
PerfuseCell prefer to pre-installed a dry stored 0.2 µm pore size SepraPor HFF.
Thalia-140 A-SUP = Alternating-Single-Use-Pump:
- Thalia keep the HFF (Hollow-Fibre-Filter) clean, free from fouling issues by programmable sequential selectable high fluid velocity flushing out debris securing optimum process time with a single HFF
- The diaphragm Alternating-Single-Use-Pump (A-SUP) exchange back and forth broth from the SUB through the HFF
- The high precision LASER sensor measures in real-time and accurate the position of the diaphragm so broth velocity and exchanged volume calculation can be performed
- Thalia TM140 exchange any volume within its spec programmable from 0.1 to +10 m/sec velocity through the HFF
- Thalia exchange equally well gas or liquids or any mix
SUS - Single-Use-Sensor's to choose from:
- CellRetention™ features pre-installed Hamilton SUS for DO, pH and level/ground as standard
- Also available with InCyte bio-mass SUS mounted vertical at the vessel wall
- SUS combines with most PCS or Cronus-PCS and Lucullus
- Completely eliminate the need for components sterilized in conventional autoclave
A-SUP – Thalia operated by the Clotho and Lachesis Drive Unit:
- CellRetention™ P-SUB works with any PCS
- Drive Unit Lachesis designed to control the Thalia Single-Use-Pump and ensure efficient and reproducible operation
- From Clotho / Lachesis programmable broth / retentate velocity and volume through HFF module for harvest and for removal of potential membrane deposits
- Connection to pressurized air and vacuum within your lab - like vacuum from Alagonia-50
- Super compact Clotho for smaller P-SUBs and Lachesis Drive Unit for larger P-SUBs