SUBmerged™ ATF P-SUB keep the HFF free from fouling
This unique 3.2 liter bioreactor operate as Perfusion-SUBmerged (P-SUB) after the CellRetention ATF method.
Benefits of the P-SUBmerged are:
- avoid HFF fouling – freedom to select broth velocities for gentle harvest and sequential pulses flushing out debris from inside the HFF
- eliminate dead volume – skip all the hoses, traditional plastic connectors and SUBmerge the HFF
- avoid cell death - inverted Thalia pump arranged on top of the HFF = hereby the diaphragm pump cannot accumulate cells
- reduce shear force – protect cells by eliminating hoses, fittings - SUBmerge the HFF inside the P-SUB and the inverted Thalia on top of HFF
SUBmerged 3 MBD+C112+TM80 Straight
SUBmerged 3 TM80+SepraPor C112
SUBmerged 3 in HSF+motor+InCyte
Left photo - The re-invented 3 liter P-SUB with SUBmerged SepraPor C112 and inverted Thalia TM80 with red LASER support on top. Centre photo - The InCyte biomass SUS patch mounted on the vessel wall and InCyte ARC sensor clicked on. Right photo - 3 liter P-SUB is mounted in the Heating-Support-Foot (HSF) on the Magnetic-Stirrer-Table (MST). Reason is simple – there is not space for both servo motor, sensors and Thalia TM80 on the OD137 mm head plate so Magnetic-Bottom-Drive (MBD) required.
P-SUBmerged 3.2 liter Vessel Volume features:
- 0.4 - 2.5 liter Working Volume (WV) fully pre-assembled and ready for use with selectable impellers of which Power Numbers are known
- Thalia diaphragm Alternating-Single-Use-Pump (A-SUP) straight or angled version integrating a
- SepraPor C112-xx1x HFF (Hollow-Fibre-Filter) with 420 cm2 surface SUBmerged directly into the broth
- Single-Use-Sensors (SUS) for DO, pH, bio-mass, temp well, level as needed
CellRetention 3 P-SUB ATF HSF MST
CellRetention 3 P-SUB+ATF+HSF+MST
CellRetention-3 B-SUB SUJ
Rendered SolidWorks CAD drawings show different details of the 3 liter P-SUBmerged all 3 driven from bottom. Left P-SUB arranged in the Heating-Support-Foot on MST incl InCyte opposite. Center - the 3 liter P-SUB surrounded by the Single-Use-Jacket (SUJ) for liquid thermal control. Right - the P-SUB incl SUJ illustrating how liquid thermal control require liquid inlet at bottom and outlet opposite at top.
Thermal control as needed - electrical or liquid with Magnetic-Bottom-Drive (MBD) or Head-Plate-Drive (HPD) - choose one of these options:
- Electrical-Heating-Blankets (EHB) (InCyte possible - require EHB attachments do not interfere)
- Electrical via Heating-Support-Foot (HSF) (InCyte possible) - see the 3rd photo
- Liquid RUJ - P-SUBmerged to be inserted into the Re-Useable-Jacket (RUJ) (InCyte not possible)
- Liquid SUJ - equipped with Single-Use-Jacket (SUJ) (InCyte not possible)
SUBmerged 3 SepraPor+Angled Thalia
RUJ for 3 liter P-SUB Stainless Steel HPD
OD137 P-SUB with EHB 75x350
If Head-Plate-Drive (HPD) is the desired option – not the safest option for a lightweight P-SUB with a heavy servo motor on top – though if the P-SUBmerged require liquid thermal control balance is obtained. If the P-SUB is inserted into the Re-Useable-Jacket (RUJ) safety is obtained. The P-SUB mounted in a 15 mm deep mechanical support on MST and wrapped with the 75 x 350 mm EHB.
SUBmerged 3 liter ATF HFF Cronus
CellRetention-3 P-SUB Thalia Straight
SUBmerged SepraPor Thalia Angled
FlowChart connects the P-SUBmerged to the more advanced Cronus-PCS running Lucullus SCADA software. Rendered CAD illustrate the 3 liter SUBmerged with straight Thalia for MST drive and with angled Thalia for HPD drive. Both designed for HSF thermal control allowing InCyte is installed.
IPR covered by: CellRetention, CerPhragm, SUBmerged.