Perfusion by CellRetention™ in ATF mode

Revolutionizing scalable Perfusion-SUBmerged™ – series 31

CellRetention™ in the SUBmerged™ design cultivate mammalian cell’s suspended at high cell density via cell retention in safe and trustworthy ATF perfusion mode operation for as long as needed. 

Benefits of P-SUBmerged are:

  • HFF fouling – freedom to select accurately any broth velocity for harvest and cleaning sequentially flushing out debris inside HFF lumen
  • Dead volume – skip all the hoses, traditional connectors, fittings and reduce dead volume to zero
  • Cell death - brand new inverted Thalia™ diaphragm pump above the HFF and P-SUB cannot accumulate cells 
  • Shear force – remove all hoses, fittings and SUBmerge the HFF directly in the broth inside the P-SUB protecting fragile cells lines

Trust your perfusion process flushing out debris accumulated inside the HFF lumens

 

3 Liter SubmergedCellvessel 500 Ml ATFDual 250 Ml + ClothoDual 250 Ml P SUB KitSubmerged 3 Liter In HSFSubmerged 13 Liter + Motor

CellRetention by Perfusion-SUBmerged (P-SUBs) are fully pre-assembled with SepraPor® Hollow-Fiber-Filter (HFF) cartridges and the Thalia diaphragm Alternating-Single-Use-Pump (A-SUP) driving the perfusion process safe. The solution is backed up by unique Clotho or Lachesis or Atropos Drive Units and software.

A new era of customizable Perfusion-Single-Use-Bioreactor's (P-SUBs) with the SUBmerged SepraPor® Hollow-Fiber-Filter (HFF) manufactured by Meissner® Corp integrated with the high precision Thalia diaphragm Alternating-Single-Use-Pump (A-SUP) and Single-Use-Sensors (SUS) from Hamilton®.

CellRetention portfolio integrated into the revolutionizing SUBmerged™ standard products right out of the box:

  • 80 - 150 ml P-SUB Working Volume (WV) - 250 ml VV - for Modular
  • 80 - 200 ml P-SUB Working Volume (WV) - 300 ml VV - for DASbox
  • 100 - 350 ml P-SUB Working Volume (WV) - 500 ml VV - for Cronus-PCS or most PCS
  • 500 - 1.5 liter P-SUB Working Volume (WV) - 2 liter VV - for Cronus-PCS or most PCS
  • 0.5 - 2 liter P-SUB Working Volume (WV) - 3 liter VV - for Cronus-PCS or most PCS
  • 2 - 5 liter P-SUB Working Volume (WV) - 6 liter VV - for Cronus-PCS or most PCS
  • 2 - 10 liter P-SUB Working Volume (WV) - 13 liter VV - for Cronus-PCS or most PCS
  • 4 - 25 liter P-SUB Working Volume (WV) - 30 liter VV - ready Q1 2026 - for Cronus-PCS

SUB (rigid PC), SUP, HFF, SUS integrated into one P-SUB unit, pre-packed and E-beam irradiated, all single-use (of course) and ready for easy use. Work with most Process-Control-Systems (PCS) combined with Clotho, Lachesis or Atropos Drive Unit manufactured by sister company www.cronus-pcs.com 

Thalia diaphragm A-SUP size TM200 and TM300 combined with SepraPor® HFF series 500 and 700 for Process Intensification:

  • 100 - 250 liter foil bag Single-Use-Bioreactor + Thalia diaphragm pump + SepraPor HFF - planned 2027
  • 500 liter foil bag Single-Use-Bioreactor + Thalia diaphragm pump + SepraPor HFF - planned 2028

 

Patents granted for CellMembra™, CerPhragm™, CellRetention™ and SUBmerged™ patent applied for. Patents and applications which gives PerfuseCell Freedom-to-Operate. Check out status here.

© 2025 PerfuseCell-Cronus A/S