SUBmerged kit fit 250 ml to 5 liter Vessel Volume STR or SUB
Any batch Stirred-Tank-Reactor (STR) or Single-Use-Bioreactor (SUB) for Process Development (PD) of mAbs supplied with a free PG13.5 port can be upgraded to perfusion SUBmerged for expressing mAbs. The presented revolutionizing DIY kit allows any one with the need and a hood to upgrade - no autoclave needed.
Check out - a 1 liter BioBlu 1c OD 110 mm - a 3 liter CellReady OD 138 mm - a 1 liter glass/steel OD110 mm STR - all 3 mounted with a SUBmerged kit unifying SepraPor OD12 x 300 mm 170 cm2 HFF with Thalia TM60A Single-Use-Pump (SUP). Easily installed at end-user site, converts the STR / SUB to a perfusion P-STR / P-SUB when one PG13.5 port is free. Angled Thalia SUP eliminates conflicts with servo motor mounted on head plate. The kit include needed dual M-flex hoses for separate required HFF wetting and permeate harvest.
SepraPor OD12 mm HFF, supplied by Meissner, will be available in length 200 mm (110 cm2 surface area) 300 mm (170 cm2 surface area) and 600 mm (340 cm2 surface area) unified with angled Thalia TM60-A SUP. Angled SUP gives space on a crowded head plate to sensors and servo motor. The Perfusion-SUBmerged ATF kits are designed as single-use, delivered dual bag packed and E-beam irradiated. And as such not designed to be autoclaved but installed to the SUB / STR under the hood. Don't forget to pre-wet the HFF.
these lines are copied from the first page and just as relevant ..............
- avoid HFF fouling – freedom to select any broth velocity flushing out debris from inside the HFF by LASER guided Thalia diaphragm pump
- avoid dead volumes – skip hoses, fittings - SUBmerged SepraPor HFF integrated with Thalia diaphragm pump eliminates dead volumes
- avoid cell death - innovative inverted Thalia pump arranged above the SUBmerged SepraPor so the Thalia pump cannot accumulate cells
- reduce shear stress – eliminating hoses, fittings and HFF SUBmerged into the broth reduces shear stress to lowest possible
- SUBmerged - HFF cartridge directly in the broth inside the SUB - no hoses, fittings needed = simplified, reliable and ready
Clotho Drive Unit is required and integrates pressures, vacuum, volume sensing eliminating the need for external sensors for HFF DeltaP and mass-flow measurements. Clotho process software controls accurately the programmable stroke volume, number of strokes, the allowed DeltaP along the HFF for fully automatic broth low harvest velocity and fully selectable broth high velocity for the sequential lumen cleaning eliminating HFF blockage.
IPR covered by: CellRetention, CerPhragm, SUBmerged.