Revolutionizing ATF-SUBmerged DIY kits – series 33

Convert STRs or SUBs into a perfusion bioreactor on your own

 

World only ATF perfusion DIY kit anyone can install and thus create the revolutionizing SUBmerged™ for Process Development. Based on a variety of existing STR (Stirred-Tank-Reactor) and SUB (Single-Use-Bioreactor) sizes from 0.250 to 5 liter Vessel Volume (VV) with one free PG13.5 port. Like for cultivating suspended cells lines reliable at high density in ATF-SUBmerged mode for expressing mAbs or proliferation of stem cells. Unique SepraPor OD12 mm HFF, pore size 0.2 µm available in 110, 170, 340 cm2 surface area for these applications unified with Thalia TM60 SUP.

 Benefits of these SUBmerged ATF kits are:

• avoid fouling – freedom to select accurately any broth velocity for gentle harvest and sequential cleaning flushing out HFF debris 
• avoid dead volume – skip conventional HFF hoses, traditional connectors, troublesome fittings - simplify and eliminate dead volume
• avoid cell death - innovative inverted LASER guided Thalia™ diaphragm pump arranged above HFF cannot accumulate cells 
• reduce shear force stress – remove HFF hoses, fittings and HFF SUBmerge directly into the broth protecting fragile cell lines      

                  Check out P-SUB product matrix under support 

 

Clotho Drive Unit is required and integrates pressures, vacuum, volume sensing eliminating the need for external sensors for HFF pressure drop, mass-flow measurements. Clotho process software master accurately the programmable each stroke volume, number of strokes, the allowed pressure drop along the HFF for fully automatic broth low harvest velocity and fully selectable higher broth velocity for the sequential lumen cleaning eliminating HFF blockage.