SUBmerged™ 250 kit for the Modular PCS
- Revolutionizing CellRetention P-SUB-250 ATF integrating SepraPor® HFF (Hollow-Fibre-Filter) into the SUB eliminates dead volumes
- Direct coupled HFF to inverted innovative LASER guided Thalia diaphragm Single-Use-Pump reduce shear stress, eliminate cell accumulation and well known HFF fouling
CellRetention 250 ml P-SUBmerged unique arrangement integrates all 3 components in one unit for very easy Modular PCS installation. Modular takes care of agitation, aeration, pH, DO and media. Clotho drive unit controls Thalia which takes care of ATF perfusion and protects the HFF.
Clotho Drive Unit integrates pressures, vacuum, volume sensing eliminating the need for external sensors for TMP, pressure drop, mass-flow measurements. Clotho process software controls accurately the programmable stroke volume, number of strokes, the allowed pressure drop along the HFF for fully automatic broth low harvest velocity and fully selectable broth high velocity for the sequential lumen cleaning eliminating HFF blockage.
Left photo shows the Modular kit ready for installation. Center photo a short Modular with two ATF SUBmerged™. Right photo shows the 250 ml ATF P-SUB vacuum packed.Combine the 250 ml "Easy-Connect" SUB with our SUBmerged kit integrating
- Combine the 250 ml "Easy-Connect" SUB with our SUBmerged kit integrating SepraPor™ 110 cm2, 0.2 µm HFF (manufactured by Meissner Corp) inserted through the SUB sample port and inverted Thalia TM50 SUP on top of HFF for trouble free installation and operation.
- Thalia™ LASER guided Single-Use-Pump controlled by outstanding Clotho™ and its software
- Alternating broth volume and velocity fully programmable, accurately measured and fully repeatable – no process guessing
- SUBmerged™ and Direct-Fit HFF eliminating shear stress caused by conventional Luer-Loc™ fittings
- The complete and pre-assembled P-SUBmerged kit packed in dual film bags and precision irradiated – easy to install the kit under the hood
- Desired hose and length for permeate and media addition as needed
Installation guide (inside the hood) is this simple - remove the 20 mm sample port plug, insert the white silicone plug into the sample port, add a suitable drop of liquid to the HFF as lubricant and push the HFF through the silicone plug to 10-15 mm above SUB bottom - that’s it !!
It is possible to operate the 250 ml SUB in Modular the old fashion way – yes, but …
Design issues for decades have been cell accumulated in the pump arranged traditionally below the HFF with huge dead volumes. Lack of variable broth velocity caused fouling issue difficult to solved. Thalia LASER guided diaphragm Single-Use-Pump is designed to play with variable broth volume and variable controlled velocity.
IPR covered by: CellRetention, CerPhragm, SUBmerged.