Large CellRetention keep HFF free from fouling

World only ready to use P-SUB for ATF method offer:

• Alternating of broth by Thalia-M140 diaphragm Alternating-Single-Use-Pump (A-SUP)
• Meissner HFF (Hollow-Fibre-Filter) with from 3,600 to 9,200 cm2 surface area fouling free process with innovative diaphragm pump
• Up to 25 liter Working Volume fully pre-assembled P-SUB

Benefits are

• HFF fouling – freedom to select broth velocities flushing out debris from inside the lumen
• Dead volume – skip all the hoses and plastic connectors
• Cell death - inverted Thalia pump above the HFF and P-SUB cannot accumulate cells 
• Shear force – remove all hoses and fittings and SUBmerge the HFF into the broth inside the P-SUB

BIG

SUBmerged 30 liter + motor

Industry leading architecture P-SUB pre-installed with SUBmerged SepraPor C324 or C424 HFF (Hollow-Fibre-Filter) directly into the broth. Ground-breaking Laser controlled Thalia diaphragm Alternating-Single-Use-Pump (A-SUP).

SUB - for cultivation

• 100% configurable design for the 30 liter Vessel Volume (VV) - Working Volume (WV) as low as 5 liter in an OD 250 x 600 mm PC vessel – sensor and impeller dependent
• Agitation from top (HPD) or from bottom by Magnetic-Bottom-Drive (MBD) driven by the Magnetic-Stirrer-Table (MST) with any type and number of impellers

SUB - Thermal Control

• Electrical-Heating-Blanker (EHB) - wrap orange silicone EHB around the P-SUB
• Liquid Single-Use-Jacket (SUJ) integrating the P-SUB inside the SUJ for HPD

HFF – harvest semi-continuous a cell free permeate

• PerfuseCell as standard integrate TM140 with a dry stored 0.2 µm pore size SepraPor HFF model C324-xx3x or C424-xx4x of 600 mm length with 2,300 cm2 or 8,500 cm2 surface area. Other requirements ask us. Only one HFF is needed - parallel HFF setup not needed as the A-SUP keep the HFF clean.

Thalia TM140 A-SUP = Alternating-Single-Use-Pump

• The diaphragm Alternating-Single-Use-Pump (A-SUP) exchange back and forth broth from the SUB directly through the HFF
• The high precision LASER sensor measures in real-time and accurate the position of the diaphragm so broth velocity and exchanged volume calculation can be performed
• Thalia TM140 will exchange any volume within its spec programmable from 0.1 to +10 m/sec velocity through the HFF flushing out fouling securing the HFF stay clean throughout the process

SUS - Single-Use-Sensor's to choose from

• CellRetention™ features pre-installed 420 mm long SUS for DO, pH, level/ground as standard
• Also available with InCyte bio-mass SUS patch mounted horizontal at the bottom of the SUB
• SUS completely eliminate the need for components sterilized in conventional autoclave

BIG

SUBmerged ATF HFF HPD Cronus

 From left seen InCyte patch installed on SUB and the InCyte ARC modules attached. Center shows some of the 600 different impellers to choose from. Left an example of a SUB with an extensive assembly of hoses and bottles.

A-SUP – Thalia operated by the Lachesis Drive Unit

• CellRetention™ P-SUBmerged and Lachesis Drive Unit works with any PCS over OPC-UA and software like Lucullus and DeltaV
• Drive Unit Lachesis designed to control the Thalia A-SUP ensure efficient and with reproducible operation
• From Lachesis programmable broth/retentate velocity and volume through HFF module for harvest and for removal of potential membrane deposits
• Connection to pressurized air and vacuum within your lab