SUBmerged™ P-SUB keep the HFF free from fouling
The 13 liter Perfusion-SUBmerged (P-SUB) is fully assembled, ready to use, operating after the ATF method.
The re-invented P-SUB for ATF method offer:
- SUBmerged SepraPor C224 HFF in a fouling free operation integrated with innovative LASER guided diaphragm pump
- 2 - 10 liter Working Volume fully pre-assembled P-SUB
- Alternating broth by Thalia-M100 diaphragm Alternating-Single-Use-Pump (A-SUP)
Benefits are:
- HFF fouling – freedom to select broth velocities flushing out debris from inside the lumen
- Dead volume – skip all the hoses and plastic connectors
- Cell death - inverted Thalia pump above the HFF and P-SUB cannot accumulate cells
- Shear force – remove all hoses and fittings and SUBmerge the HFF into the broth inside the P-SUB
The SUBmerged OD200 mm 13 liter P-SUP with TM100 mounted inverted on the SepraPor C224 HFF. The red LASER sensor for real-time measuring the position of the diaphragm for unique operation. Hereby full control of velocity and selected exchange volume. Full kit of P-SUBmerged 13 with InCyte bio-mass, VisiFerm and Oneferm.
SUB - for cultivation (the SUB is sourced from sister company CerCell)
- 100% configurable design for the 13 liter Vessel Volume (VV) - Working Volume (WV) as low as 2-4 liter in a OD 200 x 445 mm PC vessel
- Agitation from bottom by Magnetic-Bottom-Drive (MBD) driven by the Magnetic-Stirrer-Table (MST)
- Agitation from top, Head-Plate-Drive (HPD) and any type and number of impellers as an option
SUB - Thermal Control
- Electrical blanket heating - wrap orange silicone blanket around
- Electrical Jacket heating combined with with Magnetic-Bottom-Drive - metal foot also for stability
- Water Re-Usable-Jacket (RUJ) for heating/cooling even combined with Magnetic-Bottom-Drive (MST)
HFF – harvest semi-continuous a cell free permeate
PerfuseCell as standard integrate TM100 with a dry stored 0.2 µm pore size SepraPor HFF model C224-xx2x 600 mm long with 1,500 cm2 surface area.
Thalia TM100 A-SUP = Alternating-Single-Use-Pump
- The diaphragm Alternating-Single-Use-Pump (A-SUP) exchange back and forth broth from the SUB through the HFF at selected velocities
- The high precision LASER sensor measures in real-time and accurate the position of the diaphragm so broth velocity and exchanged volume calculation can be calculated
- Thalia TM100 or the TM140 will exchange any volume within its spec programmable from 0.1 to >10 m/sec velocity through the HFF securing the HFF stay clean throughout the process
SUS - Single-Use-Sensor's to choose from
- SUBmerged CellRetention™ features pre-installed 420 mm long SUS for DO, pH, level/ground as standard
- Also available with InCyte bio-mass SUS mounted horizontal at the bottom of the 13 liter SUB vessel
- Completely eliminate the need for further components added / sterilized in conventional autoclave
From left FlowwChart on SUB and the InCyte ARC modules attached. Center shows some of the 600 different impellers to choose from. Left an example of a SUB with an extensive assembly of hoses and bottles.
A-SUP – Thalia operated by both Clotho or Lachesis Drive Unit
- CellRetention™ P-SUB and Clotho (or Lachesis) works with any PCS over OPC-UA and software like Lucullus, DeltaV and DASware
- Drive Unit Clotho and Lachesis designed to control the Thalia Single-Use-Pump ensure efficient and reproducible operation
- From SUP driver programmable broth/retentate velocity and volume through HFF module for harvest and for removal of potential membrane deposits
- Connection to pressurized air and vacuum within your lab