Bio-mass and viability - not presently available
Capacitance sensor technology measures equally well adherent or suspension type of mammalian cells during cultivation. Typical range for mammalian is 105 cells/ml to 109 cells/ml. The FUTURA pico bio-mass Single-Use-Sensor' are pre-installed in the CellVessel 500 ml SUBs and as to such NO hood work is required.
The bio-mass sensor system sense total capacitance measured in pico Farad/cm with 2-3% accuracy. Viable Cell Density (VCD) system monitors the volume of live cells. The principle is expressed in permittivity as the system can detect the response of cells with intact membranes. Cell fragments, particles and gas bubbles are not measured.
The once available green FUTURA pico pre-amplifier and as installed in CellVessel 500 ml – check out more details and spec on www.futura-pico.com - biomass Single-Use-Sensor for small Single-Use-Bioreactor's is by far the wildest topic.
Measured parameters on mammalian cell (app 18 µm size) are: Viable Cell Density, Conductivity, Fc, indicating average cell size, Alpha, indicating cell size distribution, DeltaEpsilon, 50 kHz to 20 MHz multi frequency cell density. Pico connects to the Futura pre-amplifies mounted on top of the PICO SUS which connect to modules offering 4-20 mA, Modbus and Ethernet connection for integration which your PCS.
Download here detailed information. Get Aber pre-amplifier parts from the local Aber distributor.
