Parallel operating mini Perfusion-SUB for TFF method offer:
- SepraPor HFF (Hollow-Fibre-Filter) cleaned in fouling free process with innovative diaphragm pump
- 100 – 200 ml Working Volume fully pre-assembled P-SUB
- Circulation of broth by Clio CM80 diaphragm OneWay-Single-Use-Pump (O-SUP)
General features of CellMembra™ 300 mini P-SUB (ver-10) designed for DASbox
- Based on the BioBLU 0.3 batch SUB designed with magnetic HPD for the DASbox
- Fully programmable re-circulated broth volume and velocity, accurately measured and repeatable – no guessing
- SepraPor® HFF with pore 0.2 µm, 1.0 mm straw/lumen, 110 cm2 area, barb side ports
- The pre-assembled P-SUB packed in dual film bags and precision irradiated – forget the autoclave
- P-SUB dim OD 76 x 120 mm for Working Volume (WV) ranging from 80 ml up to 200 ml
- Thermal control, agitation, aeration, etc as offered by DASbox
- Clotho drive unit operate the Clio diaphragm pump and communicate via OPC-UA to DASbox
Left photo shows BioBLU 0.3 SUB converted to CellMembra-300 P-SUB incl Maxon motor. Center photo one CellMembra-300 (ver-10) with the blue head plate and visible the latest Clio CM80 SUP. Right photo of CellMembra-300 illustrating the in SUP integrated SepraPor HFF eliminating Luer-Loc hereby reducing Luer-Loc causing significant shear force.
The CellMembra-300-DASbox is delivered dual bag packed and E-beam irradiated. Heavily equipped with an extensive hose assembly ready to install and use. Example of how CellMembra-300 P-SUBs in parallel inserted into the DASbox (originally designed for OD 76 mm STR).
Flow-Chart illustrates the setup with Clotho Tower (in the yellow box) operating the LASER controlled Clio SUP. Clotho keep an eye on the TMP and ensure regular cleaning of the HFF. Center photo 8 P-SUBs in parallel. operated, controlled by dual Clotho towers and DASware or directly from the Clotho software independent of DASware. The pressurized air and vacuum delivered by dual Alagonia and one Sarpedon for each tower. A photo of a lot in production.