Control of two Clio SUP integrated with CellMembra 

Clotho is a dual channel Drive Unit for two independently operating Clio diaphragm Single-Use-Pump's integrated with CellMembra when cultivating mammalian cells in a continuous process.

Product, model name



Dual SUP drive

Supply gas pressure (dry air)

>1,1 - 5 Bar atmospheric

Supply gas vacuum 

<0,2 Bar absolute

Power supply

24 VDC, >1,5 amp

Hephaestus cabinet size


Weight, kilo



Clotho-2 is a super compact Drive Unit maximizing the performance of the Clio Single-Use-Pumps.

Clio is integrated with the Perfusion-Single-Use-Bioreactor (P-SUB's) for high cell density cultivation operating in perfusion mode. Such as the CellMembra (mini and medium) product available by PerfuseCell. The P-SUB integrate the Clio diaphragm Single-Use-Pump (SUP) the Cross-Flow-Filter (CFF) the Single-Use-Sensor's (SUS) - all in one package ready to use. Clotho control accurately the pumped volume and the velocity of the broth passing through the CFF.

Driving Clio SUP's requires pressurized air and vacuum. The SUP drive gas pressure is continuously adjusted by sets of proportional valves inside Clotho. The intelligent microprocessor with advanced PID algorithms get information from Laser and pressure sensors.

Front panel of Clotho-2 stereo/dual channel model in Hephaestus Stainless Steel cabinet shown as rendered picture with installation info. In the middle photo shown as the real Drive Unit including the two red Laser sensor's. Further Clotho-2 assembled with one Sarpedon reservoir and two Alagonia pumps in one compact tower. This fully independent setup ready to use right out of the box.


When Clotho is connected to gas supply from both a pressure and vacuum source with sufficient capacity, then the spec is:

Drive of two Clio SUP cell's

  • Pumped volume (Cardiac Output/CO) according to stroke length and cell size
  • Stroke length, %: 10 – 100 in 10% jumps
  • Clotho inform about a velocity according to CFF info input, m/s: 0,1 – 15

SUP cell size, name



Stroke Volume, SV, mL/100 % stroke



Cardiac Output, CO range, mL/min

0 - 750

0 - 1.500

Supply gas is defined as: 1 - "Supply gas pressure" and; 2 - "Supply gas vacuum". Sufficient supply is when the volume (measure in "normal liter/min") of both pneumatic pressure and vacuum quality is 1-2 times the planned CO in "nl" at specified pressures. 

Suitable supply gas pump's, also in Hephaestus cabinet's are Alagonia. Check also out the Sarpedon reservoir stabilizing pressure/vacuum and reduce pulsation when several Clio SUPs operate on the same source of supply gas.

Clotho can off course be driven by in lab installed pneumatic air and vacuum.


  • Internal 5” touch sensitive TFT display or external to PADs or smartPhones via Wi-Fi for programming and info from the built-in webserver
  • USB port for USB adapter/Wi-Fi connection 
  • RJ45 for IP/TCP via LAN and ModBus / OPC communication