Clotho-1

Drive of one Clio or Thalia

Clotho is a single channel Drive Unit for; one Clio diaphragm One-way-Single-Use-Pump (O-SUP) integrated with the CellMembra P-SUB; or one Thalia diaphragm Alternating-Single-Use-Exchanger (A-SUE) integrated with the CellRetention P-SUB. Both for cultivating mammalian cells in a semi Continuous Process (CP).

Product, model name

Clotho-1

Purpose, drive of

Single O-SUP or A-SUE

Drive Gas delivered, max Bar

1,2 Bar atmospheric

Supply Gas pressure (dry air)

1,2 - 8 Bar atmospheric

Supply Gas vacuum 

<0,1 Bar absolute

Power supply

24 VDC, >1 amp

Hephaestus cabinet size

U2

Weight, kilo

4,5

Design

Clotho-1 is a very compact Drive Unit capable of supporting both the Clio type of One-way-Single-Use-Pump and the Thalia Alternating-Single-Use-Exchanger.

Clio and Thalia is integrated with the Single-Use-Bioreactor (SUB) for high cell density cultivation operating in perfusion mode. Such as the CellMembra or CellRetention (size mini or medium) product. The P-SUB integrates with either Clio or Thalia (O-SUP or A-SUE), the Hollow-Fiber-Filter (HFF), the Single-Use-Sensor's (SUS) - all in one package ready to use. Clotho control accurately the pumped or exchanged volume and the velocity of the feedstock passing the HFF.

Driving the diaphragm O-SUP's / A-SUE's requires pressure above and vacuum below atmospheric pressure. The drive gas pressure and mass-flow is continuously adjusted via a set of proportional valves inside Clotho. Intelligent microprocessor with advanced PID algorithms get information from Laser and pressure sensor's.

Left; Clotho-1 in U2 Hephaestus U2 Stainless Steel cabinet as rendered picture. Middle: The Clotho-1 Drive Unit as delivered with one red Laser sensor and Power Supply. Right; Typical setup with one Alagonia for supply of vacuum (or even two Alagonia pumps for both vacuum and pressure) and one Sarpedon reservoir.

Specification

When Clotho is connected to gas supply from both a pressure and vacuum source with sufficient capacity, then the spec is:

  • Drive of one Clio O-SUP or Thalia A-SUE either size 30 or size 100
  • Pumped volume (Cardiac Output, CO) according to stroke length and cell size
  • Stroke length, %: 10 – 100 in 10% jumps
  • Clotho inform continuously about velocity according to HFF spec input, m/sec: 0,1 – 10

Please check:

Supply gas is defined as: 1 - "Supply gas pressure" and; 2 - "Supply gas vacuum". Sufficient supply is when the volume (measure in "normal liter/min") of both pneumatic pressure and vacuum quality is 1-2 times the planned CO in "nl" at specified pressures. 

Suitable supply gas pump's, also in Hephaestus cabinet's are Alagonia. Check also out the Sarpedon reservoir stabilizing pressure/vacuum and reduce supply pulsation when several O-SUPs or A-SUEs operate on the same source of supply gas. 

Clotho can off course be driven by in lab installed pneumatic air and vacuum

Communication and safety

  • Internal 5” touch sensitive TFT display or external to PADs or smartPhones via Wi-Fi for programming and info from the built-in webserver
  • USB port for USB adapter/Wi-Fi connection 
  • RJ45 for IP/TCP via LAN and ModBus / OPC communication
  • Laser class 2 sensor, 650 nm wavelength, <1,2 milli Watt power, IP67 - see safety notice under Download/Manuals