80 - 200 ml Perfusion-SUB

Mini P-SUB mag-HPD with O-SUP, HFF, SUS for DASbox

BioBLU Perfusion-SUB for ATF operation method offer

  • HFF (Hollow-Fibre-Filter) fouling free process with innovative diaphragm pump
  • 80 – 200 ml Working Volume fully pre-assembled P-SUB (sensor dependent)
  • Circulation of broth by Thalia M80 diaphragm Alternating-Single-Use-Pump (A-SUP)
  • Outstanding Clotho software - stand-alone or OPC-UA connected

General features of CellRetention™ mini P-SUB for DASbox:

  • Based on the BioBLU 0.3 batch SUB designed with magnetic HPD for the DASbox
  • Alternating broth volume, number of strokes and velocity fully programmable, accurately measured and fully repeatable – no guessing
  • HFF with pore 0.2 µm x 1.0 mm straw/lumen, 88 cm2 area, Luer-Lok side ports
  • The complete and pre-assembled P-SUB unit packed in dual film bags and precision irradiated – forget the autoclave
  • P-SUB dim OD76 x 120 mm for Working Volume (WV) ranging from 80 ml up to ~200 ml
  • Thermal control, agitation, aeration, etc as offered by DASbox
  • Communication with DASware

Left photo show two standard BioBLU 0.3 batch SUBs and two converted to CellMembra-300 P-SUBs. Center photo one CellRetention-300 with the blue head plate and visible the HFF on top of Thalia M80 with LASER sensor underneath. Right photo with the white ring on the Heating-Support-Foot for the BioBLU 0.3 which then fits into the iCELLis-nano docking stations with ID110 mm HSF.

Furthermore the mini P-SUB features

  • Comes with Magnetic HPD for up to 250 RPM as to the DASbox
  • One std BioBLU pitch blade impeller
  • 3 small diameter weldable hoses length 600 mm (upgraded from the 100 mm std)
  • 1 short hose with 5 cm2 sterile filter for aeration
  • 1 short hose with swabable valve for bottom sampling
  • 1 exhaust hose with 16 cm2 sterile filter

Example of how 8 in parallel CellMembra-300 inserted into the DASbox BIOblock (for OD76 mm SUBs) can operate controlled by dual Clotho each tower and DASware via OPC-UA. The pressurized air and vacuum delivered by dual Alagonia and one Sarpedon for each of two towers.