Perfusion by CellRetention™ in ATF mode

Revolutionizing scalable Perfusion-SUBmerged™ – series 31

SUBmerged™ cultivate suspended cells at high density consistent and reliable in ATF CellRetention mode operation. 

Benefits of P-SUBmerged are:

  • avoid fouling – freedom to select accurately any broth velocity for gentle harvest and sequential cleaning flushing out debris inside the HFF
  • avoid dead volume – skip conventional HFF hoses, traditional connectors, troublesome fittings -  simplify and eliminate dead volume
  • avoid cell death - innovative inverted LASER guided Thalia™ diaphragm pump arranged above HFF so Thalia pump cannot accumulate cells 
  • eliminate shear stress – remove HFF hoses, fittings and SUBmerge the HFF directly in the broth inside the P-SUB protecting fragile cell lines

                    Flush out fouling, debris collected inside the HFF

 

3 Liter SubmergedCellvessel 500 Ml ATFDual 250 Ml + ClothoDual 250 Ml P SUB KitSubmerged 3 Liter In HSFSubmerged 13 Liter + Motor

Perfusion-SUBmerged are fully assembled P-SUBs integrating SepraPor® Hollow-Fiber-Filter (HFF) cartridges and the LASER guided Thalia diaphragm Alternating-Single-Use-Pump (A-SUP) driving the perfusion process reliable. The SUBmerged portfolio is backed by one of the 3 unique sisters - Clotho, Lachesis or Atropos - Drive Units with their unprecedented software.

The new era of Perfusion-Single-Use-Bioreactor's (P-SUBmerged) combines:

  1. PerfuseCell's™ ability to create custom-engineered Single-Use-Bioreactor (SUB) solutions for any unique challenge - integrating 
  2. Meissner® customized SepraPor® Hollow-Fiber-Filter cartridges - integrated with 
  3. PerfuseCell's high precision Thalia™ LASER guided diaphragm Alternating-Single-Use-Pump (A-SUP) - driven by
  4. PerfuseCell's Drive Unit cluster of - Clotho, Lacheis, Atropos - driving the Thalia A-SUPs - and 
  5. Hamilton® precise Single-Use-Sensors (SUS)

PerfuseCell's inovative engineered product portfolio integrates the above components into the revolutionizing SUBmerged™ range - ready for use right out of the box:

  • 80 - 150 ml P-SUB Working Volume (WV) - 250 ml VV - for Modular
  • 80 - 200 ml P-SUB Working Volume (WV) - 300 ml VV - for DASbox
  • 100 - 350 ml P-SUB Working Volume (WV) - 500 ml VV - for Cronus-PCS or most PCS
  • 500 - 1.5 liter P-SUB Working Volume (WV) - 2 liter VV - for Cronus-PCS or most PCS
  • 0.4 - 2 liter P-SUB Working Volume (WV) - 3 liter VV - for Cronus-PCS or most PCS
  • 2 - 5 liter P-SUB Working Volume (WV) - 6 liter VV - for Cronus-PCS or most PCS
  • 4 - 10 liter P-SUB Working Volume (WV) - 13 liter VV - for Cronus-PCS or most PCS
  • 4 - 25 liter P-SUB Working Volume (WV) - 30 liter VV - ready Q1 2026 - for Cronus-PCS

SUB, SUP, HFF, SUS integrated into one P-SUB, pre-packed, E-beam irradiated, all single-use (of course) and ready for easy use. SUBmerged with most Process-Control-Systems (PCS) combined with stand-alone Clotho, Lachesis or Atropos Drive Unit or a complete PCS from sister Cronus-PCS.

The larger Thalia diaphragm A-SUP size TM200 and TM300 combined with SepraPor® HFF series 500 and 700 for Process Intensification:

  • 100 - 250 liter foil bag Single-Use-Bioreactor + Thalia diaphragm pump + SepraPor HFF - in planning
  • 500 liter foil bag Single-Use-Bioreactor + Thalia diaphragm pump + SepraPor HFF - in planning

 

Patents granted for CellMembra™, CerPhragm™, CellRetention™ and SUBmerged™ patent applied for. Patents and applications which gives PerfuseCell Freedom-to-Operate. Check out status here.

© 2025 PerfuseCell-Cronus A/S