Re-Invented Perfusion-Single-Use-Bioreactor (P-SUB)

The benefits of the P-SUBmerged™ ATF are:

  • avoid HFF fouling –  freedom to select any broth velocity flushing out debris from inside the HFF by LASER guided Thalia diaphragm pump
  • avoid dead volumes – skip hoses, fittings - SUBmerged SepraPor HFF and integrated with Thalia diaphragm pump eliminates dead volumes
  • avoid cell death - innovative inverted Thalia pump arranged above the SUBmerged SepraPor so the Thalia pump cannot accumulate cells 
  • reduce shear stress – eliminating hoses, fittings and SUBmerge the cartridge into the broth reduces shear stress to lowest possible
  • all by SUBmerge and Thalia - HFF cartridge directly in the broth inside the SUB - no hoses, fittings needed = simplified, reliable and ready to use

 

250 Ml ATF P SUB For Modula 2ATF 500 Ml Clotho + HarmoniaMini P SUB ATF Bioblu 2Thalia TM200 Integrating Seprapor C512Submerged 13 Liter + Motor3 Liter Submerged

Fully assembled P-SUBmerged (series 31) with Vessel Volume (VV) ranging 250 ml for the Modular or 0.3 for DASbox or scalable further from 0.5 - 3 - 6 - 13 up to 30 liter rigid plastics - at present. As Thalia and SepraPor is scalable PerfuseCell will go further up in P-SUBmerged VV when needed.

The industry is facing the well known problem of cleaning and keep the Hollow-Fiber-Filter clean in perfusion processes. Lets face it - none of the existing technologies gave us viable solutions to these problems. A better technology and reliable platform is required.

The P-SUBmerged is based on the LASER guided diaphragm Single-Use-Pumps (A-SUP). Named Thalia designed specifically to alter accurately broth velocities flushing out HFF fouling. Constant broth velocity like from centrifugal and peristaltic pumps and non-LASER guided diaphragm pumps have proven unable to clean, keep clean, flush out HFF fouling - the challenge of the entire industry.

              PerfuseCell offer these packages - starting a new era in perfusion: 

 

  1. CellRetention™ (series 31) P-SUBmerged™ operate in ATF mode and integrate the scalable Thalia Alternating-Single-Use-Pump (A-SUP) with the scalable SepraPor® Hollow-Fiber-Filter (HFF) cartridge, and a range of Single-Use-Sensors. The unique P-SUBmerged design is pre-assembled, pre-packed and ready for easy use with Clotho or Lachesis diaphragm pump Drive Units. 

  2. CellMembra™ (series 30) P-SUBs operate in TFF mode and integrate the scalable Clio OneWay-Single-Use-Pump (O-SUP) with the SepraPor® Hollow-Fiber-Filter cartridges and multiple Single-Use-Sensors. All pre-assembled, pre-packed and ready for easy use with the diaphragm pump Drive Units. 

  3. When there is a need for Thalia A-SUP (series 32) pre-assembled with larger SepraPor HFF cartridges scaling from 50 liter and up - ask PerfuseCell

  4. Drive Units Clotho and Lachesis (series 33) for Thalia and Clio are based on groundbreaking high-tech designed software. The P-SUBmerged runs perfect stand-alone on series-33 units or on Cronus-PCS supported by Lucullus or DeltaV or DASware software.

 

Patents applied for or issued for all products - check out Intellectual Property Rights

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