The fantastic Clio diaphragm Single-Use-Pump

Clio pump features

Clio is a pulsating fluid feed pump and part of CellMembra perfusion concept. Build for super controlled operation by the advanced Clotho Drive Unit. Clotho measures accurately in real-time the free-flowing diaphragm position. Allowing Clotho programming the desired diaphragm position and function. The result is an Air-Operated-Diaphragm-positive-Displacement (AODPD) One-way-Single-Use-Pump (O-SUP) with un-parallel performance and capabilities.

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Clio technical / functionality:

  • The diaphragm Single-Use-Pump (O-SUP) integrates two passive one-way valves guiding the direction of broth flow. The valve body receives broth from the SUB and deliver feedstock to the Hollow-Fiber-Filter (HFF) module inlet after which the concentrated broth passes axially the HFF cartridge and exit as retentate to be returned to the SUB.
  • Clio O-SUP integrates with both the SUB and HFF hereby operating in a loop.
  • Clio feature the re-usable (red) Laser sensor reading through the transparent Clio dome wall.
  • The high precision Laser sensor measures the position of the diaphragm, so broth velocity and volume calculation can be performed and presented on the Clotho display in real-time.
  • Clio both pump and measures any volume ranging within its spec and from 0 to +10 m/s velocity through the Cross-Flow-Filter module.

User features:

  • Clio handle fluids = liquids and / or gases or any mix of liquid/gas
  • Clio is a true metering feed pump = Positive Displacement (PD) pump = accurate mass-flow measuring at any time
  • Clio O-SUP do NOT need any mass-flow sensor measuring the mass-flow
  • Clio easily sucks from low arranged containers
  • Clio is a low shear force design which do not damage mammalian cells
  • Clio pump do NOT need calibration like peristaltic pumps
  • Clio pump do NOT loose nano size silicone particles to the fluid like peristaltic pumps

Clio general specifications:

Product, variant names

Clio-30

Clio-100

Clio-300

Clio-500

Layout, number of diaphragms

single

single

single

single

Diaphragm diameter, mm

 62

 80

 100

 125

Diaphragm stroke, mm

 25

 39

 46

 54

Stroke Volume, SV, mL/S, 100% stroke

34

100

500

500

Cardiac Output, CO range, mL/min

0-650

0-1.300

0-3.000

0-5.000

Beats-per-Minute, BpM

0-22

0-20

0-15

0-13

Typical hose connection, ID/OD mm

4/8

8/12

12/18

12/18

SUP cell weight, grams

100

200

300

400


The basics in operation is copied from the human heart functionality:

  • Cardiac Output (CO) = total pumped volume, SUP capacity, mL/min (CO = BpM x SV)
  • Beats-per-Minute (BpM) = ranging 0 - 30 BpM, SUP diameter/stroke volume depending
  • Stroke Volume (SV) = programmable from 10 - 100% of SUP specification

Physical features

  • No expensive housing materials for traditional steam cleaning requirements
  • No mechanical rotating parts such as electrical motor, coupling, rollers or gearing
  • No moving ball valves, but simple cross-slit valves
  • All single-use plastic parts supplied irradiated – contamination not possible
  • All pumps offer infinite turn-down, turn-up scale better than 1:10.000