500 ml Perfusion-SUB

World only mini Perfusion-SUB integrating both CFF, SUS and SUP

Mini P-SUBs for cell retention in perfusion cultivation setup in a fully single-use setup. The Pulsating-Tangential-Flow (PTF) which integrate a Single-Use-Bioreactor (SUB) and a Single-Use-Pump (SUP) and Single-Use-Sensor's (SUS) and the Cross-Flow-Filter (CFF) opens up for new opportunities.

General features of CellMembra™ mini P-SUB:

  • No space consuming servo motor attached to the head plate - take advantage of Magnetic-Bottom-Drive (MBD)
  • Designed with 4 x PG 13,5 head plate ports and 6 ports for In&Out with small diameter hoses
  • Pumped re-circulated broth volume fully programmable and obtained velocity accurately measured – no guessing
  • Spectrum CFF with pore 0,2 µm, 14 x 1,0 mm straw/lumen, 75 cm2 area, Luer-Lok side ports, (D02-P20U-10-N)
  • The complete and pre-assembled P-SUB unit packed in dual film bags and precision irradiated – forget the autoclave
  • SUB dimension is OD 82 x 120 mm for Working Volume (WV) ranging from 100 ml up to 400 ml
  • Thermal control either electrical or with water - HSF available for 48 VDC, for 110 VAC, for 230 VAC, for water

Center photo as mounted in liquid heated Heating-Support-Foot (HSF-W). The HSF helps stabilize the un-balanced weight distribution and improve temperature accuracy. Red Laser sensor mounted in a Re-Usable-Bracket above the dome of the Clio-30 SUP. Clotho controller require only the cable to the Laser sensor and the blue pneumatic hose to drive the SUP.

Further P-SUB mini features:

  • Comes with Magnetic-Bottom-Drive (MBD) for up to 250 RPM for use with MST
  • One pitch blade impeller
  • 3 small diameter weldable hoses length 600 mm 
  • 1 small short hose with 5 cm2 sterile filter for aeration
  • 1 small short hose with swabable valve for bottom sampling
  • 1 exhaust hose with 16 cm2 sterile filter
  • Need for liquid temperature control = Heating-Support-Foot (HSF) pn 2262-W
  • Need for electrical temperature control = Heating-Support-Foot (HSF) pn 2262-E-???

The 4 available PG 13,5 ports are used for:

  1. One port occupied by FermProbe pH Single-Use-Sensor (SUS) S8 classical signal
  2. One port occupied by VisiWell Single-Use-Well (SUW) designed for VisiFerm (RUS) DO sensor
  3. One port free - space available sufficient for 120 mm Incyst biomass Re-Usable-Sensor
  4. Suction trunk combines both In & Out for the SUP and level sensor for PCS connection