Missing Perfusion-SUB for your in-house ATF-setup ?
Then take advantage of the CellTernate product integrating the Perfusion-Single-Use-Bioreactor (SUB) and diaphragm Alternating-Single-Use-Exchanger (A-SUE) with single-use Hollow-Fiber-Filter (HFF) and Single-Use-Sensor's (SUS) – fits straight onto the ATF-2 stainless steel “pump” foot.
The missing part - WORLD FIRST all complete pre-packed, irradiated and ready to use Perfusion-Single-Use-Bioreactor to be combined with your Repligen C-24 controller.
CellTernate-2 integrates SUB/A-SUE/HFF/SUS in one package
- 3,2 liter Vessel Volume (VV) with 6 x PG 13,5 ports
- 0,4 – 2,0 liter Working Volume (WV)
- Integrated Single-Use-Sensor - OneFerm pH and VisiWell or PolarWell for your standard DO Re-Usable-Sensor's (RUS)
- 3 free PG 13,5 port for other sensor's
- A-SUE is Aglaea-100 to be controlled by the Repligen C-24 controller
Alternating-Single-Use-Exchanger (A-SUE) driven by C-24 from Repligen®
Alternating-Single-Use-Exchanger = 2,5” sanitary flange size A(TF)-SUE (pump) connected to the HFF and the HFF to the SUB. The Aglaea-100 A-SUE fits right onto your stainless steel ATF-2 “pump” foot. The A-SUE consists of: a PolyCarbonate dome with big sanitary flange, the silicone diaphragm adhered to the 2,5” flange, one on dome top 3/4" sanitary flange.
CellTernate-2 features
- First class rigid mono component PolyCarbonate components
- Dimension: OD 137 x 270 mm vessel
- Magnetic-Bottom-Drive (MBD) for impeller integrated with Aero magnet motor
- Elephant ear / Kidney impeller, OD 60 mm, 3 blades, 45 degrees, CW rotation down flow, 55 mm from the bottom
- 1 x PG 13,5 port pre-installed with PolarWell or VisiWell for non-invasive DO RUS insert
- 1 x PG 13,5 port pre-installed with polymeric pH Single-Use-Sensor with K9 connector
- 3 x free / extra sensor ports PG 13,5
- 1 x L-sparger macro or your preferred sparger
- 1 x deep tube with 500 mm hose connected directly to HFF and Aglaea-100 A-SUE
- 6 x small inner diameter 600 mm long weldable hoses for various inlet and outlets on head plate
- 1 x bottom harvest with 600 mm long weldable hoses
- 1 x exhaust with 2 x OD 60 mm sterile filters
- 1 x port for thermo couple
- Thermal control either electrical or with water - check out under Thermal Accessories
HFF - most often used with sanitary flange
- Spectrum HFF with 0,2 µm pore, 14 x 1,0 mm straw/lumen, 290 cm2 area, Luer-Lok side ports, (T06-P20U-10-N)
- Spectrum HFF with 0,2 µm pore, 75 x 1,0 mm straw/lumen, 980 cm2 area, 3/4" TC side ports, (S04-P20U-10-N)
- GE filters
One example of supplied P-SUB hardware. HFF model T06-P20U-10-N. The A-SUE is model Aglaea-100. The trick is the single-use A-SUE replacing the Stainless Steel ATF-2 dome and diaphragm.
SUS - Single-Use-Sensor’s to choose from
- One port for either PolarWell or VisiWell Single-Use-Well (SUW) for RUS
- One port for OneFerm pH Single-Use-Sensor (SUS) S8 or VP socket, classical signal
- PICO bio-mass sensor optional installed horizontal at bottom (then no RUJ)
Needed or nice to have re-usable accessories
- Re-Usable electrical Heating-Support-Foot (HSF) to be bolted on the MST = very safe solution
- Re-Usable elastic Electrical-Heating-Blanket (EHB dim 75 x 350 mm) = traditional setup
- Magnetic-Stirrer-Table (MST) like the Compact model = any servo motor
- SUB-Support-Square (SSS) for MST = use with Electrical-Heating-Blanket
- If needed - converter kit for servo motor
- If needed - Sterile-Filter-Heater (SFH)
- Accessories to be found here - Accessory Products
Other features for the 3,2 litre P-SUB
- Fully assembled P-SUB packed in dual film bags and precision irradiated - simple use.
- Supplied with a range of Single-Use-Sensors – fast to setup.
- Limited need for assembling the perfusion concept in LAF bench – less contamination risks
- Three baffles of width 10 mm x thickness 4 mm if needed – tell us
More information about CellTernate P-SUB to be found under Support