Details to Hollow-Fiber-Filter module’s
Some hints in the use of Hollow-Fibre-Filter module’s
Hollow-Fiber-Filter (HFF) membrane module/cartridge - is a device that can serve to transfer selected gases, liquids, particles between two fluids. Often a HFF device is designed with an outer rigid wall tube cartridge equipped with tube housing covers. Inside the HFF a bundle of thin wall circular straws fabricated from porous material such as polyethersulfone. This bundle of straws is sealed, cast into both end covers separating the straw inside from the straw outside and hereby appearing as a Cross-Flow-Filter (CFF).
The HFF has technically a broth/feed inlet, a concentrate/retentate outlet and a product/permeate outlet.
HFF modules are typically based on a stack of straws with diameters of 0,5 or 1 mm. Lately also available in 2 and even 3 mm diameter for biotech purposes.
Spectrum HFF per standard installed by PerfuseCell is:
- Spectrum size D or T, material/pore size P20U - dry straw, pore size 0,2 µm (200 nm), 1 mm fiber diameter - choose the length you want
- Polyethersulfone (PES) Micro-Filtration modules made from hydrophilic PES provided dry and without preservative (like Glycerin soaked)
- Installed PES modules are ready for use right out of the dual bag package
- Circulate DI water, buffer or media after unpacking and check permeate reach 2 ml/cm2 flow rate
- Follow manufactures recommendation around pre-wetting
- See Trips & Tricks
Most popular standard asymmetric HFF cartridges for R&D work
- 1/2" and 3/4" TC (Tri-Clamp) has identical outer Ferrule dimensions, but the inside bore is different
- MLL (Male LUER) - FLL (Female LUER)
- For the Spectrum model D02 material mPES 12 fibers and material PES 14 fibers