CellRetention-medium

400 - 2.000 ml Working Volume P-SUB with A-SUE to be driven by Clotho Drive Unit

PerfuseCell offer all pre-assembled 3.200 ml Vessel Volume CellRetention-medium. SUB size OD 137 x 270 mm combined with the Thalia-100 A-SUE and Hollow-Fiber-Filter and SUS – ready to use.

From left: Illustration show fluid diagram with Magnetic-Bottom-Drive SUB, controlled diaphragm A-SUE and passive HFF. The customizable SUB suction tube connects via a hose to the HFF top and opposite of HFF to the A-SUE sanitary coupling. The A-SUE is mounted on a foot, which contain the red Laser sensor for real-time measuring the position of the diaphragm and hereby full control of velocity and selected exchange volume.

From left: Pre-assembled A-SUE with sanitary coupling and bottom of HFF visible. The pre-assembled A-SUE/HFF is mounted on the LFT-100 foot which also house the red Laser sensor for real-time measuring the position of the diaphragm. 3 – Thalia-100 A-SUE next to the 3,2 liter SUB with visible impeller and Magnetic-Bottom-Drive Aero motor for agitation.

SUB - for cultivation (the SUB is sourced from sister company CerCell):

  • 100% configurable design for the 3,2 liter Vessel Volume (VV) - Working Volume (WV) as low as 400 ml
  • Agitation from bottom by Magnetic-Bottom-Drive (MBD) driven by the Magnetic-Stirrer-Table (MST)
  • Agitation from top, Head-Plate-Drive (HPD) and any type and number of impellers as an option

SUB - Thermal Control

  • Electrical blanket heating - wrap orange silicone blanket around
  • Electrical Jacket heating combined with with Magnetic-Bottom-Drive - metal foot also for stability
  • Water Re-Usable-Jacket (RUJ) for heating/cooling even combined with Magnetic-Bottom-Drive (MST)

HFF – harvest continuous a cell free permeate

PerfuseCell as standard offer a dry stored 0,2µm pore size HFF. You can though choose from one of your preferred HFF suppliers. We pre-install whichever HFF you want. We can even install two HFF in parallel – each in series with a manual valve for choice of use.

Thalia-100 A-SUE = Alternating-Single-Use-Exchanger:

  • The diaphragm Alternating-Single-Use-Exchanger (A-SUE) exchange back and forth broth from the SUB through the HFF
  • The A-SUE fits onto the re-usable Laser-Foot-Thalia
  • The high precision Laser sensor measures in real-time and accurate the position of the diaphragm so broth velocity and exchanged volume calculation can be performed
  • Thalia-100 can exchange any volume within its spec programmable from 0,1 to +10 m/sec velocity through the HFF
  • Thalia exchange equally well gas or liquids or any mix

SUS - Single-Use-Sensor's to choose from:

  • CellRetention™ features pre-installed 225 mm long SUS for DO, pH, level/ground as standard
  • Also available with Futura PICO bio-mass SUS mounted horizontal at the bottom of the 3,2 liter SUB
  • SUS combines with most PCS cabling and the FUTURA PICO bio-mass sensor amplifier
  • Completely eliminate the need for components sterilized in conventional autoclave

From left: Relevant presentation, Cronus tower (shown Clotho-dual Drive Unit and Sarpedon) setup for drive of the A-SUE (except the PCS for control of the SUB cultivation process). Details showing the red Laser sensor in the Laser-Foot, the P-SUB mounted on MST Compact (p/n 2250-C-8-B) driven by a Kollmorgen servo motor. On top of the A-SUE visible the 14 straw HFF with sanitary coupling.

A-SUE – Thalia operated by the Clotho Drive Unit:

  • CellRetention™ P-SUB works with any PCS
  • Drive Unit Clotho designed to control the Thalia Single-Use-Exchanger and ensure efficient and reproducible operation
  • From Clotho programmable broth/retentate velocity and volume through HFF module for harvest and for removal of potential membrane deposits
  • Connection to pressurized air and vacuum within your lab - or from unit's like Alagonia and Sarpedon - if you don't have access to pressurized air and vacuum
  • Super compact Clotho available in either single or dual channel models