Some basics in the use of Hollow-Fiber-Filter's
Hollow-Fiber-Filter (HFF) module - is a device that can serve to transfer selected gases, liquids, particles across a membrane between two fluids. Most often the HFF device is designed with an outer rigid wall tube equipped with tube housing covers. Inside the HFF a bundle of thin wall membrane circular straws fabricated from porous material such as polyethersulfone. This bundle of straws is sealed, cast into both end covers separating the straws inside from the straws outside hereby creating a Cross-Flow-Filter (CFF). The HFF has technically a feed/feedstock inlet, a concentrate/retentate outlet and a product/harvest/permeate outlet.
HFF modules are typically based on a stack of lumen, straws with diameters of 0,5 or 1 mm. Lately also available in 2 and even 3 mm diameter for biotech purposes.
Hollow-Fiber-Filter (HFF) principle with bundled straws - red particles = CHO cells, blue particles = proteins passing the membrane barrier. SEM photo of a 0,4 mm straw wall section Polyethersulfone (PES). Assembly of Clio-30 O-SUP connected via Luer-Lok with the HFF ready for CellMembra installation.
Spectrum HFF per standard installed by PerfuseCell is:
- Model P20U - dry straw, pore size 0,2 µm (200 nm), 1 mm diameter
- Polyethersulfone (PES) Micro-Filtration modules made with hydrophilic PES provided dry and without preservative (like Glycerin soaked)
- Installed PES modules are ready for use out of the dual bag package
- Circulate DI water, buffer or media after unpacking and check permeate reach 2 ml/cm2 flow rate
- Follow HFF manufacture recommendations
- See Trips & Tricks