Clotho-2 software

Drive of two Clio or Thalia

Clotho is a dual channel Drive Unit for two independently operating; Clio diaphragm One-way-Single-Use-Pump's integrated with CellMembra P-SUB; or Thalia diaphragm Alternating-Single-Use-Exchanger's integrated with the CellRetention P-SUB. Both for cultivating mammalian cells in a Semi-Continuous Process.

Product, model name

Clotho-2

Purpose, drive of

Dual O-SUP and/or A-SUE

Drive Gas outlet, max Bar

1,2 Bar atmospheric

Supply Gas pressure (dry air)

>1,2 - 8 Bar atmospheric

Supply Gas vacuum 

<0,1 Bar absolute

Power supply

24 VDC, >1,5 amp

Hephaestus cabinet size

U2

Weight, kilo

4,5

Design

Clotho-2 software is loaded into the ultra compact Metis Drive Unit maximizing the performance of Clio and Thalia.

Clio and Thalia is integrated with the Single-Use-Bioreactor (SUB) for high cell density cultivation operating in perfusion mode. Such as the CellMembra or CellRetention (mini and medium) products. The P-SUBs integrates either the Clio or Thalia (O-SUP or A-SUE), the Hollow-Fiber-Filter (HFF), and the Single-Use-Sensor's (SUS) - all in one package ready to use. Clotho control accurately the pumped/exchanged volume and the velocity of the feedstock passing along the HFF.

Driving Clio or Thalia requires pressurized air and vacuum. The drive gas pressure and mass-flow is continuously adjusted by sets of proportional valves inside Clotho. The intelligent microprocessor with advanced PID algorithms get information from Laser and pressure sensors.

Front panel of Clotho-2 /dual channel model in Hephaestus Stainless Steel cabinet shown as rendered picture with installation info. In the middle photo shown as the real Drive Unit including the two red Laser sensor's. Further Clotho-2 assembled with one Sarpedon reservoir and two Alagonia pumps in one compact tower. This fully independent setup ready to use right out of the box.

Specification

When Clotho is connected to gas supply from both a pressure and vacuum source with sufficient capacity, then the spec is:

  • Drive of two Clio or Thalia similtaniously
  • Pumped volume (Cardiac Output/CO) according to stroke length and size
  • Stroke length, %: 10 – 100 in 10% jumps
  • Clotho inform about a velocity according to HFF info input, m/s: 0,1 – 10

Please check:

Supply gas is defined as: 1 - "Supply gas pressure" and; 2 - "Supply gas vacuum". Sufficient supply is when the volume (measure in "normal liter/min") of both pneumatic pressure and vacuum quality is 1-2 times the planned CO in "nl" at specified pressures. 

Suitable supply gas pump's, also in Hephaestus cabinet's are Alagonia. Check also out the Sarpedon reservoir stabilizing pressure/vacuum and reduce pulsation when several SUPs operate on the same source of supply gas.

Clotho can off course be driven by in lab installed pneumatic air and vacuum.

Communication

  • Internal 5” touch sensitive TFT display or external to PADs or smartPhones via Wi-Fi for programming and info from the built-in webserver
  • USB port for USB adapter/Wi-Fi connection 
  • RJ45 for IP/TCP via LAN and ModBus / OPC communication
  • Laser class 2 sensor, 650 nm wavelength, <1,2 milli Watt power, IP67 - see safety notice under Download/Manuals