CellTank series 34

Continuous Perfusion SUB regulating biomass with temperature

CellTank series 34 is a novel Continuous-Perfusion-Single-Use-Bioreactor (CP-SUB) designed for steady-state continuous cultivation at high density of adherent as well as suspension type of mammalian cells for expression of biologics. CellTank P-SUB is a ready-to-use, pre-assembled in a pre-sterilized package designed for biomass control by temperature regulation.

CellTank is designed for Magnetic-Stirrer-Table (MST) drive operation handled by most Process-Control-Systems (PCS).

CellTank 34 series general specification:

  • Scaffold, matrix either for suspension CHO or adherent CHO cells manufactured from PET
  • Polyamide cover integrated 100% fluid tight with vessel
  • PMP (Polymethylpentene) conical griffin beaker vessel
  • Polyamide reactor body integrating envelopes filled with polyester fibre matrix for enhanced biocompatibility cultivating either adherent or suspension cell lines
  • 4-pole permanent magnet operated polyamide high performance centrifugal pump facilitating simple operation on clock-wise rotation MST
  • Drive by PCS >50 watt servo motor via Magnetic-Stirrer-Table (MST) (MST from www.cercell.com)
  • Efficient elimination of bubble and cell contact and kLa values above 40 sparging with air
  • Very efficient head space to liquid surface contact for high efficient CO2 control
  • Delivered high precision E-beam irradiated in dual polyester foil bags

CellTank model, p/n

34-0150

Vessel volume/size, ml

2,000

Media reservoir volume, ml

1,000

Working matrix volume, ml

150

Matrix surface area (for adherent CHO) cm2

36x10E+03

Required MST operation range, rpm

250 - 650

Required power delivered of MST, watt

>25

Vessel OD for heating blanket, mm

132

Weight, grams

750

CellTank facilitates the use of PG 13,5 x 120 mm classical format sensors:

  1. One pre-installed classical signal and type of pH sensor from Hamilton - included
  2. One pre-installed classical signal non-invasive VisiWell with dO2 sensor spot from Hamilton (Visiferm not included)
  3. Extra port for possible glucose and lactate measurements – not included
  4. One port designed for biomass sensor – Hamilton style Re-Usable-Sensors (RUS) – install yourself under sterile conditions – not included

All CellTank further integrate the following sensors:

  • Non-invasive temperature well for <ID6 mm sensors – facilitate your PCS standard probe for on-line measurements
  • internal media re-circulation mass flow – SUS – manual read-out of 0.4 - 2.4 litre/min
  • matrix pressure drop – SUS – manual read-out of 0 – 600 mm WC
  • media level with an insulated SS electrode in 1,300 ml height with the biomass sensor as ground

CellTank 34 available since 2013.

Check out more information under Support and under Download