CellCompact series 33

Micro and Macro perfusion SUB

CellCompact 33-01xx is a novel Micro-Perfusion-Bioreactor

The MPB kit is based on 1 ml matrix volume originally designed for work at the cell lab at KTH in Stockholm - www.biotech.kth.se. For testing and screening purposes of adherent as well as suspension type of micro-organisms, mammalian cells, insect cells in scaffoldings, matrixes, micro carriers, etc. Laminar media flow across the ~ 18 mm wide x 18 mm long x 1-3 mm thick matrix body being visible from both sides through transparent walls. To be used in combination with a peristaltic pump for media re-circulation. Samples are obtainable before and after and by flushing cavities in the porous matrix.

One kit consists of the following parts connected in series:

  • One or more MPB bodies manufactured from polycarbonate and assembled with silicone
  • 1 ml MPB of CellCore scaffoldings ranging selectable 50-200 µm pore or your preferred scaffolding, micro carriers, coating, etc.
  • silicone tubing
  • pumping tubing for 5-10 ml/min media flow capacity (equivalent to 0-10 cm/min flux)
  • one one-way valve or hose clamp allowing bio mass to be removed from the MPB (large Luer-Lok syringe not supplied)
  • Luer-Lok syringe sampling via swabable valves (small Luer-Lok syringe not supplied)
  • Standard CellVessel 21-0250 STR with support for three classical sensors (not supplied)
  • Supplied E-beam irradiated in dual bags for single-use application 

Typical usage:

  1. Micro scale perfusion cultivation accumulating expressed product into the STR (>50 ml working volume)
  2. Micro scale perfusion cultivation allowing proliferation or bleeding of cells sequentially to be drawn manually from the MPB with a syringe

 General comments and suggestions:

  • Mount the light weight STR in Heating-Support-Foot (HSF not part of kit) on a low power stirrer table with heating and temperature control
  • STR working volume range from 50-250 ml volume in the 500 ml SUB

 CellCompact 33-15xx is a Macro-Perfusion-Bioreactor

The kit is based on 15 ml cylindrical matrix volume being OD 32x18 mm. Designed for testing of adherent, suspension type of mammalian cells, insect cells in order to; 1. Locate the optimum scaffolding, matrix, micro-carrier support for CellTank, 2. Small scale perfusion in general. CellCompact kits used in combination 

Check out more information under Support